Outputs (17)

Evaluation of a Synthetic Retinoid, Ellorarxine, in the NSC-34 Cell Model of Motor Neuron Disease (2024)
Journal Article
Escudier, O., Zhang, Y., Whiting, A., & Chazot, P. (2024). Evaluation of a Synthetic Retinoid, Ellorarxine, in the NSC-34 Cell Model of Motor Neuron Disease. International Journal of Molecular Sciences, 25(18), Article 9764. https://doi.org/10.3390/ijms25189764

Amyotrophic lateral sclerosis (ALS) is the most common motor neuron disease worldwide and is characterized by progressive muscle atrophy. There are currently two approved treatments, but they only relieve symptoms briefly and do not cure the disease.... Read More about Evaluation of a Synthetic Retinoid, Ellorarxine, in the NSC-34 Cell Model of Motor Neuron Disease.

The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria (2023)
Journal Article
Waite, R., Adams, C. T., Chisholm, D. R., Sims, C. H. C., Hughes, J. G., Dias, E., …Ambler, C. A. (2023). The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria. Frontiers in Microbiology, 14, Article 1243818. https://doi.org/10.3389/fmicb.2023.1243818

The emergence of antibiotic resistance is a growing threat to human health, and therefore, alternatives to existing compounds are urgently needed. In this context, a novel fluorescent photoactivatable diarylacetylene has been identified and character... Read More about The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria.

Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production (2022)
Journal Article
Hughes, J. G., Chisholm, D. R., Whiting, A., Girkin, J. M., & Ambler, C. A. (2023). Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production. Microscopy and Microanalysis, 29(2), 529-539. https://doi.org/10.1093/micmic/ozac040

Reactive oxygen species (ROS) are naturally produced compounds that play important roles in cell signaling, gene regulation, and biological defense, including involvement in the oxidative burst that is central to the anti-microbial actions of macroph... Read More about Bullseye Analysis: A Fluorescence Microscopy Technique to Detect Local Changes in Intracellular Reactive Oxygen Species (ROS) Production.

Structure-functional relationship of cellular retinoic acid binding proteins I and II interacting with natural and synthetic ligands (2021)
Journal Article
Tomlinson, C. W., Cornish, K. A., Whiting, A., & Pohl, E. (2021). Structure-functional relationship of cellular retinoic acid binding proteins I and II interacting with natural and synthetic ligands. Acta Crystallographica Section D: Structural Biology, 77(2), 164-175. https://doi.org/10.1107/s2059798320015247

A detailed understanding of the interactions between small-molecule ligands and their proposed binding targets is of the utmost importance for modern drug-development programs. Cellular retinoic acid-binding proteins I and II (CRABPI and CRABPII) fac... Read More about Structure-functional relationship of cellular retinoic acid binding proteins I and II interacting with natural and synthetic ligands.

Cellular localisation of structurally diverse diphenylacetylene fluorophores (2020)
Journal Article
Chisholm, D. R., Hughes, J. G., Blacker, T. S., Humann, R., Adams, C., Callaghan, D., Pujol, A., Lembicz, N. K., Bain, A. J., Girkin, J. M., Ambler, C. A., & Whiting, A. (2020). Cellular localisation of structurally diverse diphenylacetylene fluorophores. Organic and Biomolecular Chemistry, 18(45), 9231-9245. https://doi.org/10.1039/d0ob01153c

Fluorescent probes are increasingly used as reporter molecules in a wide variety of biophysical experiments, but when designing new compounds it can often be difficult to anticipate the effect that changing chemical structure can have on cellular loc... Read More about Cellular localisation of structurally diverse diphenylacetylene fluorophores.

Detection and Time-Tracking Activation of a Photosensitiser on Live Single Colorectal Cancer Cells Using Raman Spectroscopy (2020)
Journal Article
Gala De Pablo, J., Chisholm, D., Ambler, C., Peyman, S., Whiting, A., & Evans, S. (2020). Detection and Time-Tracking Activation of a Photosensitiser on Live Single Colorectal Cancer Cells Using Raman Spectroscopy. Analyst, 145(17), 5878-5888. https://doi.org/10.1039/d0an01023e

Raman spectroscopy has been used to observe uptake, metabolism and response of single-cells to drugs. Photodynamic therapy is based on the use of light, a photosensitiser and oxygen to destroy tumour tissue. Here, we used single-cell Raman spectrosco... Read More about Detection and Time-Tracking Activation of a Photosensitiser on Live Single Colorectal Cancer Cells Using Raman Spectroscopy.

Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene (2019)
Journal Article
Chisholm, D., Lamb, R., Pallett, T., Affleck, V., Holden, C., Marrison, J., O'Toole, P., Ashton, P., Newling, K., Steffen, A., Nelson, A., Mahler, C., Valentine, R., Blacker, T., Bain, A. J., Girkin, J. M., Marder, T. B., Whiting, A., & Ambler, C. A. (2019). Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene. Chemical Science, 10(17), 4673-4683. https://doi.org/10.1039/c9sc00199a

Photoactivation of photosensitisers can be utilised to elicit the production of ROS, for potential therapeutic applications, including the destruction of diseased tissues and tumours. A novel class of photosensitiser, exemplified by DC324, has been d... Read More about Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene.

Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways (2019)
Journal Article
Chisholm, D., Tomlinson, C., Zhou, G.-L., Holden, C., Affleck, V., Lamb, R., Newling, K., Ashton, P., Valentine, R., Redfern, C., Erostyak, J., Makkai, G., Ambler, C., Whiting, A., & Pohl, E. (2019). Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways. ACS Chemical Biology, 14(3), 369-377. https://doi.org/10.1021/acschembio.8b00916

Retinoids, such as all-trans-retinoic acid (ATRA), are endogenous signalling molecules derived from Vitamin A that influ-ence a variety of cellular processes through mediation of transcription events in the cell nucleus. Due to these wide-ranging and... Read More about Fluorescent retinoic acid analogues as probes for biochemical and intracellular characterization of retinoid signalling pathways.

A novel fluorescence competition assay for retinoic acid binding proteins (2018)
Journal Article
Tomlinson, C. W., Chisholm, D. R., Valentine, R., Whiting, A., & Pohl, E. (2018). A novel fluorescence competition assay for retinoic acid binding proteins. ACS Medicinal Chemistry Letters, 9(12), 1297–1300-1300. https://doi.org/10.1021/acsmedchemlett.8b00420

Vitamin A derived retinoid compounds have multiple, powerful roles in the cellular growth and development cycle, and as a result have attracted significant attention from both academic and pharmaceutical research in developing and characterizing synt... Read More about A novel fluorescence competition assay for retinoic acid binding proteins.

Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales (2017)
Journal Article
Haffez, H., Khatib, T., McCaffery, P., Przyborski, S., Redfern, C., & Whiting, A. (2018). Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales. Molecular Neurobiology, 55(3), 1942-1950. https://doi.org/10.1007/s12035-017-0440-7

All-trans retinoic acid (ATRA) plays key roles in neurogenesis mediated by retinoic acid receptors (RARs). RARs are important targets for the therapeutic regulation of neurogenesis but effective drug development depends on modelling-based strategies... Read More about Neurogenesis in Response to Synthetic Retinoids at Different Temporal Scales.