Outputs (5)

It takes a dimer to tango: Oligomeric small heat shock proteins dissociate to capture substrate (2018)
Journal Article
Santhanagopalan, I., Degiacomi, M. T., Shepherd, D. A., Hochberg, G. K., Benesch, J. L., & Vierling, E. (2018). It takes a dimer to tango: Oligomeric small heat shock proteins dissociate to capture substrate. Journal of Biological Chemistry, 293(51), 19511-19521. https://doi.org/10.1074/jbc.ra118.005421

Small heat-shock proteins (sHsps) are ubiquitous molecular chaperones, and sHsp mutations or altered expression are linked to multiple human disease states. sHsp monomers assemble into large oligomers with dimeric substructure, and the dynamics of sH... Read More about It takes a dimer to tango: Oligomeric small heat shock proteins dissociate to capture substrate.

The epilepsy-associated protein TBC1D24 is required for normal development, survival and vesicle trafficking in mammalian neurons (2018)
Journal Article
Finelli, M. J., Aprile, D., Castroflorio, E., Jeans, A., Moschetta, M., Chessum, L., Degiacomi, M. T., Grasegger, J., Lupien-Meilleur, A., Bassett, A., Rossignol, E., Campeau, P. M., Bowl, M. R., Benfenati, F., Fassio, A., & Oliver, P. L. (2019). The epilepsy-associated protein TBC1D24 is required for normal development, survival and vesicle trafficking in mammalian neurons. Human Molecular Genetics, 28(4), 584-597. https://doi.org/10.1093/hmg/ddy370

Mutations in the Tre2/Bub2/Cdc16 (TBC)1 domain family member 24 (TBC1D24) gene are associated with a range of inherited neurological disorders, from drug-refractory lethal epileptic encephalopathy and DOORS syndrome (deafness, onychodystrophy, osteod... Read More about The epilepsy-associated protein TBC1D24 is required for normal development, survival and vesicle trafficking in mammalian neurons.

Lipid binding attenuates channel closure of the outer membrane protein OmpF (2018)
Journal Article
Liko, I., Degiacomi, M. T., Lee, S., Newport, T. D., Gault, J., Reading, E., …Robinson, C. V. (2018). Lipid binding attenuates channel closure of the outer membrane protein OmpF. Proceedings of the National Academy of Sciences, 115(26), 6691-6696. https://doi.org/10.1073/pnas.1721152115

Strong interactions between lipids and proteins occur primarily through association of charged headgroups and amino acid side chains, rendering the protonation status of both partners important. Here we use native mass spectrometry to explore lipid b... Read More about Lipid binding attenuates channel closure of the outer membrane protein OmpF.

On the Effect of Sphere-Overlap on Super Coarse-Grained Models of Protein Assemblies (2018)
Journal Article
Degiacomi, M. T. (2019). On the Effect of Sphere-Overlap on Super Coarse-Grained Models of Protein Assemblies. Journal of The American Society for Mass Spectrometry, 30(1), 113-117. https://doi.org/10.1007/s13361-018-1974-2

Ion mobility mass spectrometry (IM/MS) can provide structural information on intact protein complexes. Such data, including connectivity and collision cross sections (CCS) of assemblies’ subunits, can in turn be used as a guide to produce representat... Read More about On the Effect of Sphere-Overlap on Super Coarse-Grained Models of Protein Assemblies.

Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions (2018)
Journal Article
Hochberg, G. K., Shepherd, D. A., Marklund, E. G., Santhanagoplan, I., Degiacomi, M. T., Laganowsky, A., …Benesch, J. L. (2018). Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions. Science, 359(6378), 930-935. https://doi.org/10.1126/science.aam7229

Oligomeric proteins assemble with exceptional selectivity, even in the presence of closely related proteins, to perform their cellular roles. We show that most proteins related by gene duplication of an oligomeric ancestor have evolved to avoid heter... Read More about Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions.