Outputs (7)

Wide field adaptive optics microscopy using both closed loop correction and image sharpness optimization (2011)
Presentation / Conference Contribution
Bourgenot, C., Love, G. D., Saunter, C. D., & Girkin, J. M. (2011, July). Wide field adaptive optics microscopy using both closed loop correction and image sharpness optimization. Presented at Imaging and Applied Optics, Toronto, Canada

We report on results from a wide field microscope fitted with adaptive optics. We describe results based on both image optimization (wavefront sensorless adaptive optics) and full closed loop correction.

Impact of wavefront distortion and scattering on 2-photon microscopy in mammalian brain tissue. (2011)
Journal Article
Chaigneau, E., Wright, A. J., Poland, S. P., Girkin, J. M., & Silver, R. A. (2011). Impact of wavefront distortion and scattering on 2-photon microscopy in mammalian brain tissue. Optics Express, 19(23), 22755-22774. https://doi.org/10.1364/oe.19.022755

Two-photon (2P) microscopy is widely used in neuroscience, but the optical properties of brain tissue are poorly understood. We have investigated the effect of brain tissue on the 2P point spread function (PSF2P) by imaging fluorescent beads through... Read More about Impact of wavefront distortion and scattering on 2-photon microscopy in mammalian brain tissue..

Real-time optical gating for three-dimensional beating heart imaging (2011)
Journal Article
Taylor, J., Saunter, C., Love, G., Girkin, J., Henderson, D., & Chaudhry, B. (2011). Real-time optical gating for three-dimensional beating heart imaging. Journal of Biomedical Optics, 16(11), https://doi.org/10.1117/1.3652892

We demonstrate real-time microscope image gating to an arbitrary position in the cycle of the beating heart of a zebrafish embryo. We show how this can be used for high-precision prospective gating of fluorescence image slices of the moving heart. We... Read More about Real-time optical gating for three-dimensional beating heart imaging.

Improved method for kinetic studies in microreactors using flow manipulation and noninvasive Raman spectrometry. (2011)
Journal Article
Mozharov, S., Nordon, A., Littlejohn, D., Wiles, C., Watts, P., Dallin, P., & Girkin, J. (2011). Improved method for kinetic studies in microreactors using flow manipulation and noninvasive Raman spectrometry. Journal of the American Chemical Society, 133(10), 3601-3608. https://doi.org/10.1021/ja1102234

A novel method has been devised to derive kinetic information about reactions in microfluidic systems. Advantages have been demonstrated over conventional procedures for a Knoevenagel condensation reaction in terms of the time required to obtain the... Read More about Improved method for kinetic studies in microreactors using flow manipulation and noninvasive Raman spectrometry..

Multidepth, multiparticle tracking for active microrheology using a smart camera (2011)
Journal Article
Silburn, S. A., Saunter, C. D., Girkin, J. M., & Love, G. D. (2011). Multidepth, multiparticle tracking for active microrheology using a smart camera. Review of Scientific Instruments, 82(3), https://doi.org/10.1063/1.3567801

The quantitative measurement of particle motion in optical tweezers is an important tool in the study of microrheology and can be used in a variety of scientific and industrial applications. Active microheology, in which the response of optically tra... Read More about Multidepth, multiparticle tracking for active microrheology using a smart camera.

Adaptive optics for wide-field microscopy (2011)
Presentation / Conference Contribution
Bourgenot, C., Saunter, C., Girkin, J., & Love, G. (2011, January). Adaptive optics for wide-field microscopy. Presented at Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, San Francisco, CA

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensor... Read More about Adaptive optics for wide-field microscopy.

Heart synchronization for SPIM microscopy of living zebra fish (2011)
Presentation / Conference Contribution
Taylor, J., Saunter, C., Chaudhry, B., Henderson, D., Love, G., & Girkin, J. (2011, January). Heart synchronization for SPIM microscopy of living zebra fish. Presented at Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, San Francisco, CA

We describe work on producing a selective plane illumination microscope for cardiac imaging in zebra fish embryos. The system has a novel synchronization system for imaging oscillating structures (e.g. the heart) and will have adaptive optics for ima... Read More about Heart synchronization for SPIM microscopy of living zebra fish.