All Outputs (10)

Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery (2019)
Journal Article
Wang, P., Pleskot, R., Zang, J., Winkler, J., Wang, J., Yperman, K., …Hussey, P. J. (2019). Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery. Nature Communications, 10(1), Article 5132. https://doi.org/10.1038/s41467-019-12782-6

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these prote... Read More about Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery.

Connecting membranes to the actin cytoskeleton. (2017)
Journal Article
Wang, P., Hawkins, T., & Hussey, P. (2017). Connecting membranes to the actin cytoskeleton. Current Opinion in Plant Biology, 40, 71-76. https://doi.org/10.1016/j.pbi.2017.07.008

In plants, the actin cytoskeleton plays a major role in organelle movement, cargo transport, maintaining cell polarity and controlling the morphogenesis of endomembrane systems. All of these events require a direct connection between membrane structu... Read More about Connecting membranes to the actin cytoskeleton..

NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction (2017)
Journal Article
Wang, P., & Hussey, P. (2017). NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction. Journal of Experimental Botany, 68(7), 1441-1450. https://doi.org/10.1093/jxb/erx047

In plants movement of the endoplasmic reticulum (ER) is dependent on the actin cytoskeleton. However little is known about proteins that link the ER membrane and the actin cytoskeleton. Here we identified a novel protein, NETWORKED 3B (NET3B), which... Read More about NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction.

Plant Endoplasmic Reticulum-Plasma Membrane Contact Sites (2016)
Journal Article
Wang, P., Hawes, C., & Hussey, P. (2017). Plant Endoplasmic Reticulum-Plasma Membrane Contact Sites. Trends in Plant Science, 22(4), 289-297. https://doi.org/10.1016/j.tplants.2016.11.008

The endoplasmic reticulum (ER) acts as a superhighway with multiple sideroads that connects the different membrane compartments including the ER to the plasma membrane (PM). ER–PM contact sites (EPCSs) are a common feature in eukaryotic organisms, bu... Read More about Plant Endoplasmic Reticulum-Plasma Membrane Contact Sites.

Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum–plasma membrane contact sites (2016)
Journal Article
Siao, W., Wang, P., Voigt, B., Hussey, P., & Baluska, F. (2016). Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum–plasma membrane contact sites. Journal of Experimental Botany, 67(21), 6161-6171. https://doi.org/10.1093/jxb/erw381

Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulum–plasma membrane (ER–PM) contact sites in leaf and root cells. The ER–PM localization of Arabidopsis SYT1 resembles that of the extended synaptotagmins (E-SYTs) in animal cel... Read More about Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum–plasma membrane contact sites.

Arabidopsis NAP1 regulates the formation of autophagosomes (2016)
Journal Article
Wang, P., Richardson, C., Hawes, C., & Hussey, P. (2016). Arabidopsis NAP1 regulates the formation of autophagosomes. Current Biology, 26(15), 2060-2069. https://doi.org/10.1016/j.cub.2016.06.008

The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants and animals [1 and 2]. Proteins from the SCAR/WAVE complex have been found to be membrane associated in plants [3]. Using fluor... Read More about Arabidopsis NAP1 regulates the formation of autophagosomes.

Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development (2016)
Journal Article
Wang, P., Richardson, C., Hawkins, T. J., Sparkes, I., Hawes, C., & Hussey, P. J. (2016). Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development. New Phytologist, 210(4), 1311-1326. https://doi.org/10.1111/nph.13857

The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through the plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated membrane protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 to... Read More about Plant VAP27 proteins: domain characterization, intracellular localization and role in plant development.

Interactions between plant endomembrane systems and the actin cytoskeleton (2015)
Journal Article
Wang, P., & Hussey, P. J. (2015). Interactions between plant endomembrane systems and the actin cytoskeleton. Frontiers in Plant Science, 6, Article 422. https://doi.org/10.3389/fpls.2015.00422

Membrane trafficking, organelle movement, and morphogenesis in plant cells are mainly controlled by the actin cytoskeleton. Not all proteins that regulate the cytoskeleton and membrane dynamics in animal systems have functional homologs in plants, es... Read More about Interactions between plant endomembrane systems and the actin cytoskeleton.

The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum (2014)
Journal Article
Wang, P., Hawkins, T. J., Richardson, C., Cummins, I., Deeks, M. J., Sparkes, I., …Hussey, P. J. (2014). The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum. Current Biology, 24(12), 1397-1405. https://doi.org/10.1016/j.cub.2014.05.003

The cortical endoplasmic reticulum (ER) network in plants is a highly dynamic structure, and it contacts the plasma membrane (PM) at ER-PM anchor/contact sites. These sites are known to be essential for communication between the ER and PM for lipid t... Read More about The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum.

The evolution of the actin binding NET superfamily (2014)
Journal Article
Hawkins, T., Deeks, M., Wang, P., & Hussey, P. (2014). The evolution of the actin binding NET superfamily. Frontiers in Plant Science, 5, Article 254. https://doi.org/10.3389/fpls.2014.00254

The Arabidopsis Networked (NET) superfamily are plant-specific actin binding proteins which specifically label different membrane compartments and identify specialized sites of interaction between actin and membranes unique to plants. There are 13 me... Read More about The evolution of the actin binding NET superfamily.