All Outputs (5)

VceR regulates the vceCAB drug efflux pump operon of Vibrio cholerae by alternating between mutually exclusive conformations that bind either drugs or promoter DNA. (2005)
Journal Article
Borges-Walmsley, M., Du, D., McKeegan, K., Sharples, G., & Walmsley, A. (2005). VceR regulates the vceCAB drug efflux pump operon of Vibrio cholerae by alternating between mutually exclusive conformations that bind either drugs or promoter DNA. Journal of Molecular Biology, 349(2), 387-400

VceR, a member of the TetR family of transcriptional regulators, is a repressor of the vceCAB operon, which encodes a multidrug efflux pump in Vibrio cholerae. VceR binds to a 28 bp inverted-repeat within the vceR-vceC intergenic region and is dissoc... Read More about VceR regulates the vceCAB drug efflux pump operon of Vibrio cholerae by alternating between mutually exclusive conformations that bind either drugs or promoter DNA..

The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis. (2005)
Journal Article
Sanchez, H., Kidane, D., Reed, P., Curtis, F., Cozar, M., Graumann, P., …Alonso, J. (2005). The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis. Genetics, 171, 873-883

In models of Escherichia coli recombination and DNA repair, the RuvABC complex directs the branch migration and resolution of Holliday junction DNA. To probe the validity of the E. coli paradigm, we examined the impact of mutations in DeltaruvAB and... Read More about The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis..

Conservation of RecG activity from pathogens to hyperthermophiles. (2005)
Journal Article
Wen, Q., Mahdi, A., Briggs, G., Sharples, G., & Lloyd, R. (2005). Conservation of RecG activity from pathogens to hyperthermophiles. DNA Repair, 4, 23-31

Maintaining the integrity of the genome is essential for the survival of all organisms. RecG helicase plays an important part in this process in Escherichia coli, promoting recombination and DNA repair, and providing ways to rescue stalled replicatio... Read More about Conservation of RecG activity from pathogens to hyperthermophiles..

Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange (2005)
Journal Article
Maxwell, K., Reed, P., Zhang, R., Beasley, S., Walmsley, A., Curtis, F., …Sharples, G. (2005). Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange. Proceedings of the National Academy of Sciences, 102(32), 11260-11265. https://doi.org/10.1073/pnas.0503399102

Genetic recombination in bacteriophage lambda relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by beta protein. Phage lambda encodes an additional recombinase, Orf, which participates in the early stages of r... Read More about Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange.

Evolution of a phage RuvC endonuclease for resolution of both Holliday and branched DNA junctions (2005)
Journal Article
Curtis, F., Reed, P., & Sharples, G. (2005). Evolution of a phage RuvC endonuclease for resolution of both Holliday and branched DNA junctions. Molecular Microbiology, 55(5), 1332-1345. https://doi.org/10.1111/j.1365-2958.2004.04476.x

Resolution of Holliday junction recombination intermediates in most Gram-negative bacteria is accomplished by the RuvC endonuclease acting in concert with the RuvAB branch migration machinery. Gram-positive species, however, lack RuvC, with the excep... Read More about Evolution of a phage RuvC endonuclease for resolution of both Holliday and branched DNA junctions.