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The chromosome-scale reference genome of mirid bugs (Adelphocoris suturalis) genome provides insights into omnivory, insecticide resistance, and survival adaptation

Xu, Zhongping; Wang, Guanying; Luo, Jing; Zhu, Mingju; Hu, Lisong; Liang, Sijia; Li, Bo; Huang, Xingxing; Wang, Ying; Zhang, Guangyu; Zhang, Can; Zhou, Yi; Yuan, Daojun; Chen, Taiyu; Chen, Lizhen; Ma, Weihua; Gao, Wei; Lindsey, Keith; Zhang, Xianlong; Ding, Fang; Jin, Shuangxia

The chromosome-scale reference genome of mirid bugs (Adelphocoris suturalis) genome provides insights into omnivory, insecticide resistance, and survival adaptation Thumbnail


Authors

Zhongping Xu

Guanying Wang

Jing Luo

Mingju Zhu

Lisong Hu

Sijia Liang

Bo Li

Xingxing Huang

Ying Wang

Guangyu Zhang

Can Zhang

Yi Zhou

Daojun Yuan

Taiyu Chen

Lizhen Chen

Weihua Ma

Wei Gao

Xianlong Zhang

Fang Ding

Shuangxia Jin



Abstract

Background
Adelphocoris suturalis (Hemiptera: Miridae) is a notorious agricultural pest, which causes serious economic losses to a diverse range of agricultural crops around the world. The poor understanding of its genomic characteristics has seriously hindered the establishment of sustainable and environment-friendly agricultural pest management through biotechnology and biological insecticides.

Results
Here, we report a chromosome-level assembled genome of A. suturalis by integrating Illumina short reads, PacBio, 10x Chromium, and Hi-C mapping technologies. The resulting 1.29 Gb assembly contains twelve chromosomal pseudomolecules with an N50 of 1.4 and 120.6 Mb for the contigs and scaffolds, respectively, and carries 20,010 protein-coding genes. The considerable size of the A. suturalis genome is predominantly attributed to a high amount of retrotransposons, especially long interspersed nuclear elements (LINEs). Transcriptomic and phylogenetic analyses suggest that A. suturalis-specific candidate effectors, and expansion and expression of gene families associated with omnivory, insecticide resistance and reproductive characteristics, such as digestion, detoxification, chemosensory receptors and long-distance migration likely contribute to its strong environmental adaptability and ability to damage crops. Additionally, 19 highly credible effector candidates were identified and transiently overexpressed in Nicotiana benthamiana for functional assays and potential targeting for insect resistance genetic engineering.

Conclusions
The high-quality genome of A. suturalis provides an important genomic landscape for further investigations into the mechanisms of omnivory, insecticide resistance and survival adaptation, and for the development of integrated management strategies.

Citation

Xu, Z., Wang, G., Luo, J., Zhu, M., Hu, L., Liang, S., …Jin, S. (2023). The chromosome-scale reference genome of mirid bugs (Adelphocoris suturalis) genome provides insights into omnivory, insecticide resistance, and survival adaptation. BMC Biology, 21(1), Article 195. https://doi.org/10.1186/s12915-023-01666-3

Journal Article Type Article
Acceptance Date Jul 22, 2023
Online Publication Date Sep 19, 2023
Publication Date 2023
Deposit Date Feb 14, 2024
Publicly Available Date Feb 14, 2024
Journal BMC Biology
Publisher BioMed Central
Peer Reviewed Peer Reviewed
Volume 21
Issue 1
Article Number 195
DOI https://doi.org/10.1186/s12915-023-01666-3
Keywords Cell Biology; Developmental Biology; Plant Science; General Agricultural and Biological Sciences; General Biochemistry, Genetics and Molecular Biology; Physiology; Ecology, Evolution, Behavior and Systematics; Structural Biology; Biotechnology
Public URL https://durham-repository.worktribe.com/output/2255411

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Publisher Licence URL
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Copyright Statement
© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which
permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the
original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or
other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line
to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory
regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this
licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.





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