FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorum

Wang, Ruixun; Leite, Daniel J.; Karadas, Linda; Schiffer, Philipp H.; Pechmann, Matthias

Abstract

Cell migration is a fundamental component during the development of most multicellular organisms. In the early spider embryo, the collective migration of signalling cells, known as the cumulus, is required to set the dorsoventral body axis.

Here, we show that FGF signalling plays an important role during cumulus migration in the spider Parasteatoda tepidariorum. Spider embryos with reduced FGF signalling show reduced or absent cumulus migration and display dorsoventral patterning defects. Our study reveals that the transcription factor Ets4 regulates the expression of several FGF signalling components in the cumulus. In conjunction with a previous study, we show that the expression of fgf8 in the germ-disc is regulated via the Hedgehog signalling pathway. We also demonstrate that FGF signalling influences the BMP signalling pathway activity in the region around cumulus cells.

Finally, we show that FGFR signalling might also influence cumulus migration in basally branching spiders and we propose that fgf8 might act as a chemo-attractant to guide cumulus cells towards the future dorsal pole of the spider embryo.

Citation

Wang, R., Leite, D. J., Karadas, L., Schiffer, P. H., & Pechmann, M. (2023). FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorum. Developmental Biology, 494, 35-45. https://doi.org/10.1016/j.ydbio.2022.11.009

• Journal Article Type: Article
• Acceptance Date: Nov 30, 2022
• Online Publication Date: Dec 5, 2022
• Publication Date: 2023-02
• Deposit Date: Oct 31, 2023
• Publicly Available Date: Oct 31, 2023
• Journal: Developmental Biology
• Print ISSN: 0012-1606
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 494
• Pages: 35-45
• DOI: https://doi.org/10.1016/j.ydbio.2022.11.009
• Public URL: https://durham-repository.worktribe.com/output/1872720