J.M Atkinson
Membrane Type Matrix Metalloproteinases (MMPs) Show Differential Expression in Non-small Cell Lung Cancer (NSCLC) Compared to Normal Lung: Correlation of MMP-14 mRNA Expression and Proteolytic Activity
Atkinson, J.M; Pennington, C.J; Martin, S.W; Anikin, V.A; Mearns, A.J; Loadman, P.M; Edwards, D.R; Gill, J.H
Authors
C.J Pennington
S.W Martin
V.A Anikin
A.J Mearns
P.M Loadman
D.R Edwards
J.H Gill
Abstract
Improved understanding of the involvement of matrix metalloproteinases (MMPs), including membrane-type MMPs (MT-MMPs), in human tumours has potential diagnostic, prognostic and therapeutic implications. We assessed the relationship between MT-MMP expression and clinicopathological parameters in human non-small cell lung cancer (NSCLC) and histologically normal lung tissue by quantitative Real Time PCR (qRT-PCR). All MT-MMPs (MMPs 14-17, 24 and 25) were detected by qRT-PCR with significantly higher MMP-14, -15 and -17 expression observed in tumour relative to normal lung specimens. MMP-16 was undetectable in normal lung but expressed in 8% tumours. MMP-15 demonstrated significant overexpression in adenocarcinomas relative to squamous cell carcinomas and normal lung tissue. MMP-14 mRNA expression strongly correlated to MMP-14 proteolytic activity in preclinical tumour models, indicating that qRT-PCR may predict MMP-14 activity levels in NSCLC. These data suggest that MMP-14, -15 and -17 may be good markers of disease, or therapeutic targets for treatment of human NSCLC.
Citation
Atkinson, J., Pennington, C., Martin, S., Anikin, V., Mearns, A., Loadman, P., …Gill, J. (2007). Membrane Type Matrix Metalloproteinases (MMPs) Show Differential Expression in Non-small Cell Lung Cancer (NSCLC) Compared to Normal Lung: Correlation of MMP-14 mRNA Expression and Proteolytic Activity. European Journal of Cancer, 43(11), 1764-1771. https://doi.org/10.1016/j.ejca.2007.05.009
Journal Article Type | Article |
---|---|
Publication Date | Jul 1, 2007 |
Deposit Date | Jan 7, 2013 |
Publicly Available Date | Feb 12, 2013 |
Journal | European Journal of Cancer |
Print ISSN | 0959-8049 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 43 |
Issue | 11 |
Pages | 1764-1771 |
DOI | https://doi.org/10.1016/j.ejca.2007.05.009 |
Keywords | Matrix metalloproteinases, Membrane-type metalloproteinases, Lung cancer, Real-time PCR, Carcinoma, Non-small cell lung, Cancer therapeutics. |
Public URL | https://durham-repository.worktribe.com/output/1491054 |
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Copyright Statement
NOTICE: this is the author’s version of a work that was accepted for publication in European journal of cancer. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European journal of cancer, 43/11, 2007, 10.1016/j.ejca.2007.05.009
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