Outputs (6)

High-speed dual color fluorescence lifetime endomicroscopy for highly-multiplexed pulmonary diagnostic applications and detection of labeled bacteria (2018)
Journal Article
Pedretti, E., Tanner, M. G., Choudhary, T. R., Krstajić, N., Megia-Fernandez, A., Henderson, R. K., Bradley, M., Thomson, R. R., Girkin, J. M., Dhaliwal, K., & Dalgarno, P. A. (2019). High-speed dual color fluorescence lifetime endomicroscopy for highly-multiplexed pulmonary diagnostic applications and detection of labeled bacteria. Biomedical Optics Express, 10(1), 181-195. https://doi.org/10.1364/boe.10.000181

We present a dual-color laser scanning endomicroscope capable of fluorescence lifetime endomicroscopy at one frame per second (FPS). The scanning system uses a coherent imaging fiber with 30,000 cores. High-speed lifetime imaging is achieved by distr... Read More about High-speed dual color fluorescence lifetime endomicroscopy for highly-multiplexed pulmonary diagnostic applications and detection of labeled bacteria.

Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate–evoked calcium signaling (2018)
Journal Article
Wilson, C., Lee, M. D., Heathcote, H., Zhang, X., Buckley, C., Girkin, J. M., Saunter, C. D., & McCarron, J. G. (2019). Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate–evoked calcium signaling. Journal of Biological Chemistry, 294(3), 758-758. https://doi.org/10.1074/jbc.ra118.005913

Endothelial cells are reported to be glycolytic and to minimally rely on mitochondria for ATP generation. Rather than providing energy, mitochondria in endothelial cells may act as signaling organelles that control cytosolic Ca2+ signaling or modify... Read More about Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate–evoked calcium signaling.

Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480 (2018)
Journal Article
Gala de Pablo, J., Chisholm, D. R., Steffen, A., Nelson, A. K., Mahler, C., Marder, T. B., …Evans, S. D. (2018). Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480. Analyst, 143(24), 6113-6120. https://doi.org/10.1039/c8an01461b

The development of new imaging tools, molecules and modalities is crucial to understanding biological processes and the localised cellular impact of bioactive compounds. A small molecule photosensitiser, DC473, has been designed to be both highly flu... Read More about Tandem fluorescence and Raman (fluoRaman) characterisation of a novel photosensitiser in colorectal cancer cell line SW480.

Effects of maternal anxiety and depression on fetal neuro-development (2018)
Journal Article
Reissland, N., Froggatt, S., Reames, E., & Girkin, J. (2018). Effects of maternal anxiety and depression on fetal neuro-development. Journal of Affective Disorders, 241, 469-474. https://doi.org/10.1016/j.jad.2018.08.047

Background: Fetal development is affected by maternal mental health with research indicating that maternal anxiety and depression are co-morbid; nevertheless differential effects on the fetus have been found. This study examines, prenatally, effects... Read More about Effects of maternal anxiety and depression on fetal neuro-development.

Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM (2018)
Journal Article
Young, L., Jarrin, M., Saunter, C., Quinlan, R., & Girkin, J. (2018). Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM. Biomedical Optics Express, 9(5), 2176-2188. https://doi.org/10.1364/boe.9.002176

Graded refractive index lenses are inherent to advanced visual systems in animals. By understanding their formation and local optical properties, significant potential for improved ocular healthcare may be realized. We report a novel technique measur... Read More about Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM.

The light-sheet microscopy revolution (2018)
Journal Article
Girkin, J. M., & Carvalho, M. T. (2018). The light-sheet microscopy revolution. Journal of Optics, 20(5), Article 053002. https://doi.org/10.1088/2040-8986/aab58a

This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodol... Read More about The light-sheet microscopy revolution.