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BONCAT-iTRAQ Labelling Reveals Molecular Markers of Adaptive Responses in Toxoplasma gondii to Pyrimethamine Treatment (2024)
Journal Article
Mina, J. G., Parthasarathy, A., Porta, E. O., Denny, P. W., & Kalesh, K. (2024). BONCAT-iTRAQ Labelling Reveals Molecular Markers of Adaptive Responses in Toxoplasma gondii to Pyrimethamine Treatment. Pathogens, 13(10), Article 879. https://doi.org/10.3390/pathogens13100879

We employed a BONCAT-iTRAQ labelling approach to investigate newly synthesised proteins (NSPs) in Toxoplasma gondii subjected to varying concentrations of the antifolate drug pyrimethamine. Our results reveal that numerous NSPs exhibited altered expr... Read More about BONCAT-iTRAQ Labelling Reveals Molecular Markers of Adaptive Responses in Toxoplasma gondii to Pyrimethamine Treatment.

Inhibition of HSP90 distinctively modulates the global phosphoproteome of Leishmania mexicana developmental stages (2023)
Journal Article
Porta, E. O., Gao, L., Denny, P. W., Steel, P. G., & Kalesh, K. (2023). Inhibition of HSP90 distinctively modulates the global phosphoproteome of Leishmania mexicana developmental stages. Microbiology Spectrum, 11(6), Article e02960-23. https://doi.org/10.1128/spectrum.02960-23

Heat shock protein 90 (HSP90) is an evolutionarily conserved chaperone protein that plays a central role in the folding and maturation of a large array of client proteins. In the unicellular parasite Leishmania, the etiological agent of the neglected... Read More about Inhibition of HSP90 distinctively modulates the global phosphoproteome of Leishmania mexicana developmental stages.

Quantitative Proteomics Reveals that Hsp90 Inhibition Dynamically Regulates Global Protein Synthesis in Leishmania mexicana (2021)
Journal Article
Karunakaran, K., Sundriyal, S., Perera, H., Cobb, S. L., & Denny, P. W. (2021). Quantitative Proteomics Reveals that Hsp90 Inhibition Dynamically Regulates Global Protein Synthesis in Leishmania mexicana. mSystems, 6(3), Article e00089-21. https://doi.org/10.1128/msystems.00089-21

Heat shock protein 90 (Hsp90) is a conserved molecular chaperone responsible for the folding and maturation of nascent proteins. Hsp90 is regarded as a master regulator of protein homeostasis in the cell, and its inhibition affects the functions of a... Read More about Quantitative Proteomics Reveals that Hsp90 Inhibition Dynamically Regulates Global Protein Synthesis in Leishmania mexicana.

Affinity‐based proteomics reveals novel targets of inositol pyrophosphate (5‐IP7)‐dependent phosphorylation and binding in Trypanosoma cruzi replicative stages (2021)
Journal Article
Mantilla, B. S., Karunakaran, K., Brown, N. W., Fiedler, D., & Docampo, R. (2021). Affinity‐based proteomics reveals novel targets of inositol pyrophosphate (5‐IP7)‐dependent phosphorylation and binding in Trypanosoma cruzi replicative stages. Molecular Microbiology, 115(5), 986-1004. https://doi.org/10.1111/mmi.14672

Diphosphoinositol-5-pentakisphosphate (5-PP-IP5), also known as inositol heptakisphosphate (5-IP7), has been described as a high-energy phosphate metabolite that participates in the regulation of multiple cellular processes through protein binding or... Read More about Affinity‐based proteomics reveals novel targets of inositol pyrophosphate (5‐IP7)‐dependent phosphorylation and binding in Trypanosoma cruzi replicative stages.

Defeating the trypanosomatid trio: proteomics of the protozoan parasites causing neglected tropical diseases (2020)
Journal Article
Parthasarathy, A., & Karunakaran, K. (2020). Defeating the trypanosomatid trio: proteomics of the protozoan parasites causing neglected tropical diseases. RSC Medicinal Chemistry, 11(6), 625-645. https://doi.org/10.1039/d0md00122h

Mass spectrometry-based proteomics enables accurate measurement of the modulations of proteins on a large scale upon perturbation and facilitates the understanding of the functional roles of proteins in biological systems. It is a particularly releva... Read More about Defeating the trypanosomatid trio: proteomics of the protozoan parasites causing neglected tropical diseases.

A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation (2019)
Journal Article
Karunakaran, K., & Denny, P. W. (2019). A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation. PLoS Neglected Tropical Diseases, 13(12), Article e0007651. https://doi.org/10.1371/journal.pntd.0007651

Adaptation to starvation is integral to the Leishmania life cycle. The parasite can survive prolonged periods of nutrient deprivation both in vitro and in vivo. The identification of parasite proteins synthesised during starvation is key to unravelli... Read More about A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation.

An Integrated Chemical Proteomics Approach for Quantitative Profiling of Intracellular ADP-Ribosylation (2019)
Journal Article
Karunakaran, K., Lukauskas, S., Borg, A. J., Snijders, A. P., Ayyappan, V., Leung, A. K., Haskard, D. O., & DiMaggio, P. A. (2019). An Integrated Chemical Proteomics Approach for Quantitative Profiling of Intracellular ADP-Ribosylation. Scientific Reports, 9, Article 6655. https://doi.org/10.1038/s41598-019-43154-1

ADP-ribosylation is integral to a diverse range of cellular processes such as DNA repair, chromatin regulation and RNA processing. However, proteome-wide investigation of its cellular functions has been limited due to numerous technical challenges in... Read More about An Integrated Chemical Proteomics Approach for Quantitative Profiling of Intracellular ADP-Ribosylation.

The Quest for Novel Antimicrobial Compounds: Emerging Trends in Research, Development, and Technologies (2019)
Journal Article
Mantravadi, P., Karunakaran, K., Dobson, R., Hudson, A., & Parthasarathy, A. (2019). The Quest for Novel Antimicrobial Compounds: Emerging Trends in Research, Development, and Technologies. Antibiotics, 8(1), Article 8. https://doi.org/10.3390/antibiotics8010008

Pathogenic antibiotic resistant bacteria pose one of the most important health challenges of the 21st century. The overuse and abuse of antibiotics coupled with the natural evolutionary processes of bacteria has led to this crisis. Only incremental a... Read More about The Quest for Novel Antimicrobial Compounds: Emerging Trends in Research, Development, and Technologies.

Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane (2017)
Journal Article
Clulow, J. A., Storck, E. M., Lanyon-Hogg, T., Kalesh, K. A., Jones, L. H., & Tate, E. W. (2017). Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane. Chemical Communications, 53(37), 5182-5185. https://doi.org/10.1039/c6cc08797c

Sulforaphane is a small molecule isothiocyanate which exhibits anticancer potential, yet its biological targets remain poorly understood. Here we employ a competition-based chemical proteomics strategy to profile sulforaphane’s targets and identify o... Read More about Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane.

Synthesis and Biological Evaluation of N-((1-(4-(Sulfonyl)piperazin-1-yl)cycloalkyl)methyl)benzamide Inhibitors of Glycine Transporter-1 (2016)
Journal Article
Cioffi, C. L., Liu, S., Wolf, M. A., Guzzo, P. R., Sadalapure, K., Parthasarathy, V., Loong, D. T., Maeng, J.-H., Carulli, E., Fang, X., Karunakaran, K., Matta, L., Choo, S. H., Panduga, S., Buckle, R. N., Davis, R. N., Sakwa, S. A., Gupta, P., Sargent, B. J., Moore, N. A., …Mhyre, A. J. (2016). Synthesis and Biological Evaluation of N-((1-(4-(Sulfonyl)piperazin-1-yl)cycloalkyl)methyl)benzamide Inhibitors of Glycine Transporter-1. Journal of Medicinal Chemistry, 59(18), 8473-8494. https://doi.org/10.1021/acs.jmedchem.6b00914