Skip to main content

Research Repository

Advanced Search

All Outputs (41)

The Chemical Reactivity of Membrane Lipids (2024)
Journal Article
Duché, G., & Sanderson, J. M. (2024). The Chemical Reactivity of Membrane Lipids. Chemical Reviews, 124(6), 3284-3330. https://doi.org/10.1021/acs.chemrev.3c00608

It is well-known that aqueous dispersions of phospholipids spontaneously assemble into bilayer structures. These structures have numerous applications across chemistry and materials science and form the fundamental structural unit of the biological m... Read More about The Chemical Reactivity of Membrane Lipids.

Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes (2021)
Journal Article
Esmaeil, V. S., Britt, H. M., Mosely, J. A., & Sanderson, J. M. (2021). Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes. Faraday Discussions, 2021(232), 282-294. https://doi.org/10.1039/d1fd00030f

Acyl transfer from lipids to membrane-associated peptides is a well-documented process, leading to the generation of a lipidated peptide and a lysolipid. In this article, we demonstrate that acyl transfer from lysophosphatidylcholines (lysoPCs) to th... Read More about Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes.

17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers (2020)
Journal Article
Rowlands, L. J., Marks, A., Sanderson, J. M., & Law, R. V. (2020). 17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers. Chemical Communications, 56(92), 14499-14502. https://doi.org/10.1039/d0cc05466f

Cholesterol is a crucial component of biological membranes and can interact with other membrane components through hydrogen bonding. NMR spectroscopy has been used previously to investigate this bonding, however this study represents the first 17O NM... Read More about 17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers.

Lysis of Membrane Lipids Promoted by Small Organic Molecules: Reactivity Depends on Structure but not Lipophilicity (2020)
Journal Article
Britt, H. M., Prakash, A. S., Appleby, S., Mosely, J. A., & Sanderson, J. M. (2020). Lysis of Membrane Lipids Promoted by Small Organic Molecules: Reactivity Depends on Structure but not Lipophilicity. Science Advances, 6(17), Article eaaz8598. https://doi.org/10.1126/sciadv.aaz8598

Several organic molecules of low molecular weight (<150 Da) are demonstrated to have substantial membrane-lytic potential despite having a low predicted lipophilicity (logD < 1 at neutral pH). In aqueous liposome dispersions, 38 aromatic compounds we...

Far From Inert: Membrane Lipids Possess Intrinsic Reactivity That Has Consequences For Cell Biology (2020)
Journal Article
Sanderson, J. M. (2020). Far From Inert: Membrane Lipids Possess Intrinsic Reactivity That Has Consequences For Cell Biology. BioEssays, 42(3), Article 1900147. https://doi.org/10.1002/bies.201900147

In this article, it is hypothesized that a fundamental chemical reactivity exists between some non‐lipid constituents of cellular membranes and ester‐based lipids, the significance of which is not generally recognized. Many peptides and smaller organ... Read More about Far From Inert: Membrane Lipids Possess Intrinsic Reactivity That Has Consequences For Cell Biology.

The Influence of Cholesterol on Melittin Lipidation in Neutral Membranes (2018)
Journal Article
Britt, H. M., Mosely, J. A., & Sanderson, J. M. (2019). The Influence of Cholesterol on Melittin Lipidation in Neutral Membranes. Physical Chemistry Chemical Physics, 21(2), 631-640. https://doi.org/10.1039/c8cp06661b

The effects of cholesterol on the process of intrinsic lipidation, whereby an acyl chain is transferred from a lipid as donor to a membrane-associated acceptor molecule, have been explored using melittin as the acceptor. Membranes comprising lipids w... Read More about The Influence of Cholesterol on Melittin Lipidation in Neutral Membranes.

Lytic reactions of drugs with lipid membranes (2018)
Journal Article
Britt, H. M., García-Herrero, C. A., Denny, P. W., Mosely, J. A., & Sanderson, J. M. (2019). Lytic reactions of drugs with lipid membranes. Chemical Science, 10(3), 674-680. https://doi.org/10.1039/c8sc04831b

Propranolol is shown to undergo lipidation reactions in three types of lipid membrane: (1) synthetic single-component glycerophospholipid liposomes; (2) liposomes formed from complex lipid mixtures extracted from E. coli or liver cells; and (3) in ce... Read More about Lytic reactions of drugs with lipid membranes.

The Lipidation Profile of Aquaporin-0 Correlates with The Acyl Composition of Phosphoethanolamine Lipids in Lens Membranes (2016)
Journal Article
Ismail, V. S., Mosely, J. A., Tapodi, A., Quinlan, R. A., & Sanderson, J. M. (2016). The Lipidation Profile of Aquaporin-0 Correlates with The Acyl Composition of Phosphoethanolamine Lipids in Lens Membranes. BBA - Biomembranes, 1858(11), 2763-2768. https://doi.org/10.1016/j.bbamem.2016.06.026

The lens fiber major intrinsic protein (otherwise known as aquaporin-0 (AQP0), MIP26 and MP26) has been examined by mass spectrometry (MS) in order to determine the speciation of acyl modifications to the side chains of lysine residues and the N-term... Read More about The Lipidation Profile of Aquaporin-0 Correlates with The Acyl Composition of Phosphoethanolamine Lipids in Lens Membranes.

The Association of Defensin HNP-2 with Negatively Charged Membranes: A Combined Fluorescence and Linear Dichroism Study (2016)
Journal Article
Pridmore, C. J., Rodger, A., & Sanderson, J. M. (2016). The Association of Defensin HNP-2 with Negatively Charged Membranes: A Combined Fluorescence and Linear Dichroism Study. BBA - Biomembranes, 1858(4), 892-903. https://doi.org/10.1016/j.bbamem.2016.01.014

The association of defensin HNP-2 with negatively charged membranes has been studied using a new approach that combines fluorescence and linear dichroism (LD) spectroscopies with simulated LD spectra in order to characterise the binding kinetics and... Read More about The Association of Defensin HNP-2 with Negatively Charged Membranes: A Combined Fluorescence and Linear Dichroism Study.

Optimised conditions for the synthesis of 17O and 18O labelled cholesterol (2015)
Journal Article
de la Calle Arregui, C., Purdie, J. A., Haslam, C. A., Law, R. V., & Sanderson, J. M. (2015). Optimised conditions for the synthesis of 17O and 18O labelled cholesterol. Chemistry and Physics of Lipids, 195, 58-62. https://doi.org/10.1016/j.chemphyslip.2015.12.003

Conditions are described for the preparation of cholesterol with 17O and 18O labels from i-cholesteryl methyl ether using minimal amounts of isotopically enriched water. Optimum yields employed trifluoromethanesulfonic acid as catalyst in 1,4-dioxane... Read More about Optimised conditions for the synthesis of 17O and 18O labelled cholesterol.

Phage Orf family recombinases: conservation of activities and involvement of the central channel in DNA binding (2014)
Journal Article
Curtis, F., Malay, A., Trotter, A., Wilson, L., Barradell-Black, M., Bowers, L., …Sharples, G. (2014). Phage Orf family recombinases: conservation of activities and involvement of the central channel in DNA binding. PLoS ONE, 9(8), Article e102454. https://doi.org/10.1371/journal.pone.0102454

Genetic and biochemical evidence suggests that λ Orf is a recombination mediator, promoting nucleation of either bacterial RecA or phage Redβ recombinases onto single-stranded DNA (ssDNA) bound by SSB protein. We have identified a diverse family of O... Read More about Phage Orf family recombinases: conservation of activities and involvement of the central channel in DNA binding.

Acyl Transfer from Membrane Lipids to Peptides Is a Generic Process (2013)
Journal Article
Dods, R., Bechinger, B., Mosely, J., & Sanderson, J. (2013). Acyl Transfer from Membrane Lipids to Peptides Is a Generic Process. Journal of Molecular Biology, 425(22), 4379-4387. https://doi.org/10.1016/j.jmb.2013.07.013

The generality of acyl transfer from phospholipids to membrane-active peptides has been probed using liquid chromatography–mass spectrometry analysis of peptide–lipid mixtures. The peptides examined include melittin, magainin II, PGLa, LAK1, LAK3 and... Read More about Acyl Transfer from Membrane Lipids to Peptides Is a Generic Process.

Resolving the kinetics of lipid, protein and peptide diffusion in membranes (2012)
Journal Article
Sanderson, J. M. (2012). Resolving the kinetics of lipid, protein and peptide diffusion in membranes. Molecular membrane biology, 29(5), 118-143. https://doi.org/10.3109/09687688.2012.678018

Recent developments in the understanding of molecular diffusion phenomena in membranes are reviewed. Both model bilayers and biological membranes are considered in respect of lateral diffusion, rotational diffusion and transverse diffusion (flip-flop... Read More about Resolving the kinetics of lipid, protein and peptide diffusion in membranes.

The innate reactivity of a membrane associated peptide towards lipids: acyl transfer to melittin without enzyme catalysis (2012)
Journal Article
Dods, R., Mosely, J., & Sanderson, J. (2012). The innate reactivity of a membrane associated peptide towards lipids: acyl transfer to melittin without enzyme catalysis. Organic and Biomolecular Chemistry, 10(28), 5371-5378. https://doi.org/10.1039/c2ob07113d

The innate reactivity of the peptide melittin (H-GIGAVLKVLTTGLPALISWIKRKRQQ-NH2) towards membrane lipids has been explored using LC-MS methods. The high sensitivity afforded by LC-MS analysis enabled acyl transfer to the peptide to be detected, withi... Read More about The innate reactivity of a membrane associated peptide towards lipids: acyl transfer to melittin without enzyme catalysis.

Acyl transfer from phosphocholine lipids to melittin (2011)
Journal Article
Pridmore, C. J., Mosely, J. A., Rodger, A., & Sanderson, J. M. (2011). Acyl transfer from phosphocholine lipids to melittin. Chemical Communications, 47(5), 1422-1424. https://doi.org/10.1039/c0cc04677a

Transfer of fatty acyl groups from membrane phospholipids to melittin, a commonly studied membrane-active peptide, has been observed to occur over extended time periods. Transfer can be detected after 1–2 days and selectively targets amino groups at... Read More about Acyl transfer from phosphocholine lipids to melittin.

Influence of Lipids on the Interfacial Disposition of Respiratory Syncytical Virus Matrix Protein (2011)
Journal Article
McPhee, H., Carlisle, J., Beeby, A., Money, V., Watson, M., Yeo, R., & Sanderson, J. (2011). Influence of Lipids on the Interfacial Disposition of Respiratory Syncytical Virus Matrix Protein. Langmuir, 27(1), 304-311. https://doi.org/10.1021/la104041n

The propensity of a matrix protein from an enveloped virus of the Mononegavirales family to associate with lipids representative of the viral envelope has been determined using label-free methods, including tensiometry and Brewster angle microscopy o... Read More about Influence of Lipids on the Interfacial Disposition of Respiratory Syncytical Virus Matrix Protein.