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All Outputs (14)

Exogenous abscisic acid treatment regulates protein secretion in sorghum cell suspension cultures. (2023)
Journal Article
Muthego, D., Moloi, S. J., Brown, A. P., Goche, T., Chivasa, S., & Ngara, R. (2023). Exogenous abscisic acid treatment regulates protein secretion in sorghum cell suspension cultures. Plant Signaling & Behavior, 18(1), Article 2291618. https://doi.org/10.1080/15592324.2023.2291618

Drought stress adversely affects plant growth, often leading to total crop failure. Upon sensing soil water deficits, plants switch on biosynthesis of abscisic acid (ABA), a stress hormone for drought adaptation. Here, we used exogenous ABA applicati... Read More about Exogenous abscisic acid treatment regulates protein secretion in sorghum cell suspension cultures..

Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA) (2023)
Journal Article
De-Thier, J. S., Pyati, P., Bell, J., Readshaw, J. J., Brown, A. P., & Fitches, E. C. (2023). Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA). Microbial Cell Factories, 22(1), Article 157. https://doi.org/10.1186/s12934-023-02176-1

Background
New bioinsecticides with novel modes of action are urgently needed to minimise the environmental and safety hazards associated with the use of synthetic chemical pesticides and to combat growing levels of pesticide resistance. The pea see... Read More about Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA).

Independent Membrane Binding Properties of the Caspase Generated Fragments of the Beaded Filament Structural Protein 1 (BFSP1) Involves an Amphipathic Helix (2023)
Journal Article
Jarrin, M., Kalligeraki, A. A., Uwineza, A., Cawood, C. S., Brown, A. P., Ward, E. N., Le, K., Freitag-Pohl, S., Pohl, E., Kiss, B., Tapodi, A., & Quinlan, R. A. (online). Independent Membrane Binding Properties of the Caspase Generated Fragments of the Beaded Filament Structural Protein 1 (BFSP1) Involves an Amphipathic Helix. Cells, https://doi.org/10.3390/cells12121580

Background: BFSP1 (beaded filament structural protein 1) is a plasma membrane, Aqua- 21 porin 0 (AQP0/MIP)-associated intermediate filament protein expressed in the eye lens. BFSP1 is 22 myristoylated, a post-translation modification that requires ca... Read More about Independent Membrane Binding Properties of the Caspase Generated Fragments of the Beaded Filament Structural Protein 1 (BFSP1) Involves an Amphipathic Helix.

Enhancing the oral and topical insecticidal efficacy of a commercialized spider venom peptide biopesticide via fusion to the carrier snowdrop lectin (Galanthus nivalis agglutinin) (2022)
Journal Article
Sukiran, N. A., Pyati, P., Willis, C. E., Brown, A. P., Readshaw, J. J., & Fitches, E. C. (2023). Enhancing the oral and topical insecticidal efficacy of a commercialized spider venom peptide biopesticide via fusion to the carrier snowdrop lectin (Galanthus nivalis agglutinin). Pest Management Science, 79(1), 284-294. https://doi.org/10.1002/ps.7198

BACKGROUND: Spear®-T sold as a contact foliar spray for the control of glasshouse pests such as aphids, thrips, spider mites and whiteflies, contains the recombinant spider venom peptide GS-ω/κ-HxTx-Hv1h (named as GS-ω/κ-HxTx-Hv1a by Vestaron) as the... Read More about Enhancing the oral and topical insecticidal efficacy of a commercialized spider venom peptide biopesticide via fusion to the carrier snowdrop lectin (Galanthus nivalis agglutinin).

Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome (2022)
Journal Article
Guillen-Garcia, A., Gibson, S., Jordan, C., Ramaswamy, V., Linthwaite, V., Bromley, E., …Cann, M. (2022). Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome. Nature Communications, 13, Article 5289. https://doi.org/10.1038/s41467-022-32925-6

Light harvesting is fundamental for production of ATP and reducing equivalents for CO2 fixation during photosynthesis. However, electronic energy transfer (EET) through a photosystem can harm the photosynthetic apparatus when not balanced with CO2. H... Read More about Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome.

Ubiquitin is a carbon dioxide-binding protein (2021)
Journal Article
Linthwaite, V., Pawloski, W., Pegg, H., Townsend, P., Thomas, M., Brown, A., …Cann, M. (2021). Ubiquitin is a carbon dioxide-binding protein. Science Advances, 7(39), Article eabi5507. https://doi.org/10.1126/sciadv.abi5507

The identification of CO2-binding proteins is crucial to understanding CO2-regulated molecular processes. CO2 can form a reversible posttranslational modification through carbamylation of neutral N-terminal -amino or lysine -amino groups. We have p... Read More about Ubiquitin is a carbon dioxide-binding protein.

Isolation of Arabidopsis extracellular ATP‐binding proteins by affinity proteomics and identification of PHOSPHOLIPASE C‐LIKE 1 as an extracellular protein essential for fumonisin B1 toxicity (2021)
Journal Article
Smith, S. J., Goodman, H., Kroon, J. T., Brown, A. P., Simon, W. J., & Chivasa, S. (2021). Isolation of Arabidopsis extracellular ATP‐binding proteins by affinity proteomics and identification of PHOSPHOLIPASE C‐LIKE 1 as an extracellular protein essential for fumonisin B1 toxicity. The Plant Journal, 106(5), 1387-1400. https://doi.org/10.1111/tpj.15243

ATP is secreted to the extracellular matrix where it activates plasma membrane receptors for controlling plant growth and stress‐adaptive processes. DOES NOT RESPOND TO NUCLEOTIDES 1 (DORN1), the first plant ATP receptor was identified, but key downs... Read More about Isolation of Arabidopsis extracellular ATP‐binding proteins by affinity proteomics and identification of PHOSPHOLIPASE C‐LIKE 1 as an extracellular protein essential for fumonisin B1 toxicity.

Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures (2020)
Journal Article
Ngcala, M. G., Goche, T., Brown, A. P., Chivasa, S., & Ngara, R. (2020). Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures. Proteomes, 8(4), Article 29. https://doi.org/10.3390/proteomes8040029

Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extr... Read More about Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures.

The identification of carbon dioxide mediated protein post-translational modifications (2018)
Journal Article
Linthwaite, V., Janus, J., Brown, A., Wong-Pascua, D., O’Donoghue, A., Porter, A., …Cann, M. (2018). The identification of carbon dioxide mediated protein post-translational modifications. Nature Communications, 9, Article 3092. https://doi.org/10.1038/s41467-018-05475-z

Carbon dioxide is vital to the chemistry of life processes including metabolism, cellular homoeostasis, and pathogenesis. CO2 is generally unreactive but can combine with neutral amines to form carbamates on proteins under physiological conditions. T... Read More about The identification of carbon dioxide mediated protein post-translational modifications.

SUMO Is a Critical Regulator of Salt Stress Responses in Rice (2016)
Journal Article
Srivastava, A. K., Zhang, C., Yates, G., Bailey, M., Brown, A., & Sadanandom, A. (2016). SUMO Is a Critical Regulator of Salt Stress Responses in Rice. Plant Physiology, 170, 2378-2391. https://doi.org/10.1104/pp.15.01530

SUMO (Small Ubiquitin-like Modifier) conjugation onto target proteins has emerged as a very influential class of protein modification systems. SUMO1/2 double mutant plants are nonviable, underlining the importance of SUMO conjugation to plant surviva... Read More about SUMO Is a Critical Regulator of Salt Stress Responses in Rice.

Towards the genetic engineering of triacylglycerols of defined fatty acid composition: Major changes in erucic acid content at the sn-2position affected by the introduction of a1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthesdouglasii into oi (1996)
Journal Article
Brough, C., Coventry, J., Christie, W., Kroon, J., Brown, A., Barsby, T., & Slabas, A. (1996). Towards the genetic engineering of triacylglycerols of defined fatty acid composition: Major changes in erucic acid content at the sn-2position affected by the introduction of a1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthesdouglasii into oi. Molecular Breeding, 2(2), 133-142