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Epididymal mRNA expression profiles for the protein disulfide isomerase gene family: Modulation by development and androgens. (2024)
Journal Article
Fernandes, S. G., Ferreira, L. G. A., Benham, A. M., & Avellar, M. C. W. (online). Epididymal mRNA expression profiles for the protein disulfide isomerase gene family: Modulation by development and androgens. Andrology, https://doi.org/10.1111/andr.13700

The endoplasmic reticulum (ER) is the central hub for protein quality control, where the protein disulfide isomerases (PDIs), encoded by at least 21 genes, play a pivotal role. These multifunctional proteins contribute to disulfide bond formation, pr... Read More about Epididymal mRNA expression profiles for the protein disulfide isomerase gene family: Modulation by development and androgens..

Para-Hydroxycinnamic Acid Mitigates Senescence and Inflammaging in Human Skin Models (2024)
Journal Article
Tan, C. Y. R., Morenc, M., Setiawan, M., Lim, Z. Z. Y., Soon, A. L., Bierman, J. C., Vires, L., Laughlin, T., DeAngelis, Y. M., Rovito, H., Jarrold, B. B., Nguyen, T. Q. N., Lim, J. S. Y., Kent, O., Määttä, A., Benham, A. M., Hawkins, T. J., Lee, X. E., Ehrman, M. C., Oblong, J. E., …Bellanger, S. (2024). Para-Hydroxycinnamic Acid Mitigates Senescence and Inflammaging in Human Skin Models. International Journal of Molecular Sciences, 25(15), Article 8153. https://doi.org/10.3390/ijms25158153

Para-hydroxycinnamic acid (pHCA) is one of the most abundant naturally occurring hydroxycinnamic acids, a class of chemistries known for their antioxidant properties. In this study, we evaluated the impact of pHCA on different parameters of skin agin... Read More about Para-Hydroxycinnamic Acid Mitigates Senescence and Inflammaging in Human Skin Models.

Inhibition of PDIs Downregulates Core LINC Complex Proteins, Promoting the Invasiveness of MDA-MB-231 Breast Cancer Cells in Confined Spaces In Vitro (2024)
Journal Article
Young, N., Gui, Z., Mustafa, S., Papa, K., Jessop, E., Ruddell, E., …Karakesisoglou, I. (2024). Inhibition of PDIs Downregulates Core LINC Complex Proteins, Promoting the Invasiveness of MDA-MB-231 Breast Cancer Cells in Confined Spaces In Vitro. Cells, 13(11), Article 906. https://doi.org/10.3390/cells13110906

Eukaryotic cells tether the nucleoskeleton to the cytoskeleton via a conserved molecular bridge, called the LINC complex. The core of the LINC complex comprises SUN-domain and KASH-domain proteins that directly associate within the nuclear envelope l... Read More about Inhibition of PDIs Downregulates Core LINC Complex Proteins, Promoting the Invasiveness of MDA-MB-231 Breast Cancer Cells in Confined Spaces In Vitro.

A Potential Role of Keratinocyte-Derived Bilirubin in Human Skin Yellowness and Its Amelioration by Sucrose Laurate/Dilaurate (2022)
Journal Article
Fang, B., Card, P. D., Chen, J., Li, L., Laughlin, T., Jarrold, B., …Hakozaki, T. (2022). A Potential Role of Keratinocyte-Derived Bilirubin in Human Skin Yellowness and Its Amelioration by Sucrose Laurate/Dilaurate. International Journal of Molecular Sciences, 23(11), Article 5884. https://doi.org/10.3390/ijms23115884

Sallow and/or dull skin appearance is greatly attributable to the yellow components of skin tone. Bilirubin is a yellow chromophore known to be made in the liver and/or spleen and is transported throughout the body via the blood stream. Recent public... Read More about A Potential Role of Keratinocyte-Derived Bilirubin in Human Skin Yellowness and Its Amelioration by Sucrose Laurate/Dilaurate.

Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro (2021)
Journal Article
Hunter-Featherstone, E., Young, N., Chamberlain, K., Cubillas, P., Hulette, B., Wei, X., …Karakesisoglou, I. (2021). Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro. Cells, 10(5), https://doi.org/10.3390/cells10051177

Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond to mechanical cues by altering their proliferation, migration, and... Read More about Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro.

Elucidation of the AGR2 interactome in esophageal adenocarcinoma cells identifies a redox sensitive chaperone hub for the quality control of MUC-5AC (2019)
Journal Article
Worfolk, J., Bell, S., Simpson, L., Carne, N., Francis, S., Engelbertsen, V., …Benham, A. (2019). Elucidation of the AGR2 interactome in esophageal adenocarcinoma cells identifies a redox sensitive chaperone hub for the quality control of MUC-5AC. Antioxidants and Redox Signaling, 31(15), 1117-1132. https://doi.org/10.1089/ars.2018.7647

Aims: AGR2 is a tissue-restricted member of the protein disulfide isomerase family that has attracted interest because it is highly expressed in a number of cancers, including gastroesophageal adenocarcinoma. The behavior of AGR2 was analyzed under o... Read More about Elucidation of the AGR2 interactome in esophageal adenocarcinoma cells identifies a redox sensitive chaperone hub for the quality control of MUC-5AC.

Reductive stress selectively disrupts collagen homeostasis and modifies growth factor-independent signalling through the MAPK/Akt pathway in human dermal fibroblasts (2019)
Journal Article
Carne, N., Brown, A., Bell, S., Maatta, A., Flagler, M., & Benham, A. (2019). Reductive stress selectively disrupts collagen homeostasis and modifies growth factor-independent signalling through the MAPK/Akt pathway in human dermal fibroblasts. Molecular and Cellular Proteomics, 18(6), 1123-1137. https://doi.org/10.1074/mcp.ra118.001140

Redox stress is a well-known contributor to ageing and diseases in skin. Reductants such as dithiothreitol (DTT) can trigger a stress response by disrupting disulfide bonds. However, the quantitative response of the cellular proteome to reductants ha... Read More about Reductive stress selectively disrupts collagen homeostasis and modifies growth factor-independent signalling through the MAPK/Akt pathway in human dermal fibroblasts.

Endoplasmic Reticulum redox pathways: in sickness and in health (2018)
Journal Article
Benham, A. M. (2019). Endoplasmic Reticulum redox pathways: in sickness and in health. The FEBS Journal, 286(2), 311--321. https://doi.org/10.1111/febs.14618

The Endoplasmic Reticulum (ER) is the major site for secretory protein production in eukaryotic cells and like an efficient factory, it has the capacity to expand or contract its output depending on the demand for its services. A primary function of... Read More about Endoplasmic Reticulum redox pathways: in sickness and in health.

GnRH immunization alters the expression and distribution of protein disulfide isomerases in the epididymis (2016)
Journal Article
Schorr-Lenz, A., Alves, J., Hence, N., Seidel, P., Benham, A., & Bustamante-Filho, I. (2016). GnRH immunization alters the expression and distribution of protein disulfide isomerases in the epididymis. Andrology, 4(5), 957-963. https://doi.org/10.1111/andr.12205

Hypogonadism is defined as the inadequate gonadal production of testosterone. Low serum testosterone leads to infertility by impairing spermatogenesis and reducing sperm count, however, the impact of hypogonadism in epididymal sperm maturation is poo... Read More about GnRH immunization alters the expression and distribution of protein disulfide isomerases in the epididymis.

Calreticulin is required for development of the cumulus oocyte complex and female fertility (2015)
Journal Article
Tokuhiro, K., Satouh, Y., Nozawa, K., Isotani, A., Fujihara, Y., Hirashima, Y., …Ikawa, M. (2015). Calreticulin is required for development of the cumulus oocyte complex and female fertility. Scientific Reports, 5, https://doi.org/10.1038/srep14254

Calnexin (CANX) and calreticulin (CALR) chaperones mediate nascent glycoprotein folding in the endoplasmic reticulum. Here we report that these chaperones have distinct roles in male and female fertility. Canx null mice are growth retarded but fertil... Read More about Calreticulin is required for development of the cumulus oocyte complex and female fertility.

Platinum(II) Complexes of N^C^N‑Coordinating 1,3-Bis(2-pyridyl)benzene Ligands: Thiolate Coligands Lead to Strong Red Luminescence from Charge-Transfer States (2014)
Journal Article
Tarran, W., Freeman, G., Murphy, L., Benham, A., Kataky, R., & Williams, J. (2014). Platinum(II) Complexes of N^C^N‑Coordinating 1,3-Bis(2-pyridyl)benzene Ligands: Thiolate Coligands Lead to Strong Red Luminescence from Charge-Transfer States. Inorganic Chemistry, 53(11), 5738-5749. https://doi.org/10.1021/ic500555w

A new family of platinum(II) complexes of the form PtLnSR have been prepared, where Ln represents a cyclometalating, NCN-bound tridentate ligand and SR is a monodentate thiolate ligand. The complexes fall into two groups, those of PtL1SR where HL1 =... Read More about Platinum(II) Complexes of N^C^N‑Coordinating 1,3-Bis(2-pyridyl)benzene Ligands: Thiolate Coligands Lead to Strong Red Luminescence from Charge-Transfer States.

Expression of the Endoplasmic Reticulum Oxidoreductase Ero1α in gastro-intestinal cancer reveals a link between homocysteine and oxidative protein folding. (2013)
Journal Article
Battle, D., Dias-Gunasekara, S., Watson, G., Mohamed Ahmed, E., Saysell, C., Altaf, N., …Benham, A. (2013). Expression of the Endoplasmic Reticulum Oxidoreductase Ero1α in gastro-intestinal cancer reveals a link between homocysteine and oxidative protein folding. Antioxidants and Redox Signaling, 19(1), 24-45. https://doi.org/10.1089/ars.2012.4651

Ero1-PDI interactions, the response to redox flux and the implications for disulfide bond formation in the mammalian endoplasmic reticulum (2013)
Journal Article
Benham, A., van Lith, M., Sitia, R., & Braakman, I. (2013). Ero1-PDI interactions, the response to redox flux and the implications for disulfide bond formation in the mammalian endoplasmic reticulum. Philosophical Transactions of the Royal Society B: Biological Sciences, 368(1617), Article 20110403. https://doi.org/10.1098/rstb.2011.0403

The protein folding machinery of the endoplasmic reticulum (ER) ensures that proteins entering the eukaryotic secretory pathway acquire appropriate post-translational modifications and reach a stably folded state. An important component of this prote... Read More about Ero1-PDI interactions, the response to redox flux and the implications for disulfide bond formation in the mammalian endoplasmic reticulum.

Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility (2012)
Journal Article
Tokuhiro, K., Ikawa, M., Benham, A., & Okabe, M. (2012). Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility. Proceedings of the National Academy of Sciences, 109(10), 3850-3855. https://doi.org/10.1073/pnas.1117963109

A disintegrin and metalloproteinase 3 (ADAM3) is a sperm membrane protein critical for both sperm migration from the uterus into the oviduct and sperm primary binding to the zona pellucida (ZP). Here we show that the testis-specific protein disulfide... Read More about Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility.

Conserved cysteine residues in the mammalian lamin A tail are essential for cellular responses to ROS generation (2011)
Journal Article
Pekovic, V., Gibbs-Seymour, I., Markiewicz, E., Alzoghaibi, F., Benham, A., Edwards, R., …Hutchison, C. (2011). Conserved cysteine residues in the mammalian lamin A tail are essential for cellular responses to ROS generation. Aging Cell, 10(6), 1067-1079. https://doi.org/10.1111/j.1474-9726.2011.00750.x

Pre-lamin A and progerin have been implicated in normal aging, and the pathogenesis of age-related degenerative diseases is termed ‘laminopathies’. Here, we show that mature lamin A has an essential role in cellular fitness and that oxidative damage... Read More about Conserved cysteine residues in the mammalian lamin A tail are essential for cellular responses to ROS generation.

Fertilization: a sperm's journey to and interaction with the oocyte. (2010)
Journal Article
Ikawa, M., Inoue, N., Benham, A., & Okabe, M. (2010). Fertilization: a sperm's journey to and interaction with the oocyte. Journal of Clinical Investigation, 120(4), 984-994. https://doi.org/10.1172/jci41585

Mammalian fertilization comprises sperm migration through the female reproductive tract, biochemical and morphological changes to sperm, and sperm-egg interaction in the oviduct. Recent gene knockout approaches in mice have revealed that many factors... Read More about Fertilization: a sperm's journey to and interaction with the oocyte..