Lydia Costello
Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct
Costello, Lydia; Goncalves, Kirsty; De Los Santos Gomez, Paola; Hulette, Ben; Dicolandrea, Teresa; Flagler, Michael J; Isfort, Robert; Oblong, John; Bascom, Charlie; Przyborski, Stefan
Authors
Kirsty Goncalves kirsty.e.goncalves@durham.ac.uk
Post Doctoral Research Associate
Paola De Los Santos Gomez paola.de-los-santos-gomez@durham.ac.uk
Academic Visitor
Ben Hulette
Teresa Dicolandrea
Michael J Flagler
Robert Isfort
John Oblong
Charlie Bascom
Professor Stefan Przyborski stefan.przyborski@durham.ac.uk
Deputy Provost
Abstract
Increased prevalence of skin ageing is a growing concern due to an ageing global population and has both sociological and psychological implications. The use of more clinically predictive in vitro methods for dermatological research is becoming commonplace due to initiatives and the cost of clinical testing. In this study, we utilise a well-defined and characterised bioengineered skin construct as a tool to investigate the cellular and molecular dynamics involved in skin ageing from a dermal perspective. Through incorporation of ageing fibroblasts into the dermal compartment we demonstrate the significant impact of dermal-epidermal crosstalk on the overlying epidermal epithelium. We characterise the paracrine nature of dermal-epidermal communication and the impact this has during skin ageing. Soluble factors, such as inflammatory cytokines released as a consequence of senescence associated secretory phenotype (SASP) from ageing fibroblasts, are known to play a pivotal role in skin ageing. Here, we demonstrate their effect on epidermal morphology and thickness, but not keratinocyte differentiation or tissue structure. Through a novel in vitro strategy utilising bioengineered tissue constructs, this study offers a unique reductionist approach to study epidermal and dermal compartments in isolation and tandem.
Citation
Costello, L., Goncalves, K., De Los Santos Gomez, P., Hulette, B., Dicolandrea, T., Flagler, M. J., Isfort, R., Oblong, J., Bascom, C., & Przyborski, S. (online). Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct. Journal of Cellular Physiology, https://doi.org/10.1002/jcp.31463
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 30, 2024 |
Online Publication Date | Oct 8, 2024 |
Deposit Date | Oct 8, 2024 |
Publicly Available Date | Oct 16, 2024 |
Journal | Journal of Cellular Physiology |
Print ISSN | 0021-9541 |
Electronic ISSN | 1097-4652 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1002/jcp.31463 |
Keywords | inflammation, dermal‐epidermal crosstalk, human skin equivalent, bioengineering, extracellular matrix, ageing |
Public URL | https://durham-repository.worktribe.com/output/2950174 |
Publisher URL | https://onlinelibrary.wiley.com/journal/10974652 |
Files
Published Journal Article (Advance Online Version)
(3.2 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
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