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Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct

Costello, Lydia; Goncalves, Kirsty; De Los Santos Gomez, Paola; Hulette, Ben; Dicolandrea, Teresa; Flagler, Michael J; Isfort, Robert; Oblong, John; Bascom, Charlie; Przyborski, Stefan

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Authors

Lydia Costello

Ben Hulette

Teresa Dicolandrea

Michael J Flagler

Robert Isfort

John Oblong

Charlie Bascom



Abstract

Increased prevalence of skin ageing is a growing concern due to an ageing global population and has both sociological and psychological implications. The use of more clinically predictive in vitro methods for dermatological research is becoming commonplace due to initiatives and the cost of clinical testing. In this study, we utilise a well-defined and characterised bioengineered skin construct as a tool to investigate the cellular and molecular dynamics involved in skin ageing from a dermal perspective. Through incorporation of ageing fibroblasts into the dermal compartment we demonstrate the significant impact of dermal-epidermal crosstalk on the overlying epidermal epithelium. We characterise the paracrine nature of dermal-epidermal communication and the impact this has during skin ageing. Soluble factors, such as inflammatory cytokines released as a consequence of senescence associated secretory phenotype (SASP) from ageing fibroblasts, are known to play a pivotal role in skin ageing. Here, we demonstrate their effect on epidermal morphology and thickness, but not keratinocyte differentiation or tissue structure. Through a novel in vitro strategy utilising bioengineered tissue constructs, this study offers a unique reductionist approach to study epidermal and dermal compartments in isolation and tandem.

Citation

Costello, L., Goncalves, K., De Los Santos Gomez, P., Hulette, B., Dicolandrea, T., Flagler, M. J., Isfort, R., Oblong, J., Bascom, C., & Przyborski, S. (online). Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct. Journal of Cellular Physiology, https://doi.org/10.1002/jcp.31463

Journal Article Type Article
Acceptance Date Sep 30, 2024
Online Publication Date Oct 8, 2024
Deposit Date Oct 8, 2024
Publicly Available Date Oct 16, 2024
Journal Journal of Cellular Physiology
Print ISSN 0021-9541
Electronic ISSN 1097-4652
Publisher Wiley
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1002/jcp.31463
Keywords inflammation, dermal‐epidermal crosstalk, human skin equivalent, bioengineering, extracellular matrix, ageing
Public URL https://durham-repository.worktribe.com/output/2950174
Publisher URL https://onlinelibrary.wiley.com/journal/10974652

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