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MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast

Twell, D.; Park, S.K.; Hawkins, T.J.; Schubert, D.; Schmidt, R.; Smertenko, A.; Hussey, P.J.

Authors

D. Twell

S.K. Park

D. Schubert

R. Schmidt

A. Smertenko



Abstract

MOR1 is a member of the MAP215 family of microtubule-associated proteins and is required to establish interphase arrays of cortical microtubules in plant cells1. Here we show that MOR1 binds microtubules in vivo, localizing to both cortical microtubules and to areas of overlapping microtubules in the phragmoplast. Genetic complementation of the cytokinesis-defective gemini pollen 1-1 (gem1-1) mutation with MOR1 shows that MOR1 (which is synonymous with the protein GEM1) is essential in cytokinesis. Phenotypic analysis of gem1-1 and gem1-2, which contains a T-DNA insertion, confirm that MOR1/GEM1 is essential for regular patterns of cytokinesis. Both the gem1-1 and gem1-2 mutations cause the truncation of the MOR1/GEM1 protein. In addition, the carboxy-terminal domain of the protein, which is absent in both mutants, binds microtubules in vitro. Our data show that MOR1/GEM1 has an essential role in the cytokinetic phragmoplast.

Journal Article Type Article
Publication Date Sep 1, 2002
Deposit Date May 17, 2007
Journal Nature Cell Biology
Print ISSN 1465-7392
Electronic ISSN 1476-4679
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 4
Issue 9
Pages 711-714
DOI https://doi.org/10.1038/ncb844
Public URL https://durham-repository.worktribe.com/output/1585000