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Extracellular ATP functions as an endogenous external metabolite regulating plant cell viability

Chivasa, S; Ndimba, BK; Simon, JW; Lindsey, K; Slabas, AR


BK Ndimba

JW Simon

AR Slabas


ATP is a vital molecule used by living organisms as a universal source of energy required to drive the cogwheels of intracellular biochemical reactions necessary for growth and development. Animal cells release ATP to the extracellular milieu, where it functions as the primary signaling cue at the epicenter of a diverse range of physiological processes. Although recent findings revealed that intact plant tissues release ATP as well, there is no clearly defined physiological function of extracellular ATP in plants. Here, we show that extracellular ATP is essential for maintaining plant cell viability. Its removal by the cell-impermeant traps glucose–hexokinase and apyrase triggered death in both cell cultures and whole plants. Competitive exclusion of extracellular ATP from its binding sites by treatment with ß,-methyleneadenosine 5'-triphosphate, a nonhydrolyzable analog of ATP, also resulted in death. The death response was observed in Arabidopsis thaliana, maize (Zea mays), bean (Phaseolus vulgaris), and tobacco (Nicotiana tabacum). Significantly, we discovered that fumonisin B1 (FB1) treatment of Arabidopsis triggered the depletion of extracellular ATP that preceded cell death and that exogenous ATP rescues Arabidopsis from FB1-induced death. These observations suggest that extracellular ATP suppresses a default death pathway in plants and that some forms of pathogen-induced cell death are mediated by the depletion of extracellular ATP.


Chivasa, S., Ndimba, B., Simon, J., Lindsey, K., & Slabas, A. (2005). Extracellular ATP functions as an endogenous external metabolite regulating plant cell viability. The Plant Cell, 17(11), 3019-3034.

Journal Article Type Article
Publication Date Nov 1, 2005
Deposit Date May 17, 2007
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 17
Issue 11
Pages 3019-3034