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The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling

Chilley, P.M.; Casson, S.A.; Tarkowski, P.; Wang, K.L.-C.; Hawkins, N.; Hussey, P.J.; Beale, M.; Ecker, J.R.; Sandberg, G.K.; Lindsey, K.

Authors

P.M. Chilley

S.A. Casson

P. Tarkowski

K.L.-C. Wang

N. Hawkins

M. Beale

J.R. Ecker

G.K. Sandberg



Abstract

The rate and plane of cell division and anisotropic cell growth are critical for plant development and are regulated by diverse mechanisms involving several hormone signaling pathways. Little is known about peptide signaling in plant growth; however, Arabidopsis thaliana POLARIS (PLS), encoding a 36–amino acid peptide, is required for correct root growth and vascular development. Mutational analysis implicates a role for the peptide in hormone responses, but the basis of PLS action is obscure. Using the Arabidopsis root as a model to study PLS action in plant development, we discovered a link between PLS, ethylene signaling, auxin homeostasis, and microtubule cytoskeleton dynamics. Mutation of PLS results in an enhanced ethylene-response phenotype, defective auxin transport and homeostasis, and altered microtubule sensitivity to inhibitors. These defects, along with the short-root phenotype, are suppressed by genetic and pharmacological inhibition of ethylene action. PLS expression is repressed by ethylene and induced by auxin. Our results suggest a mechanism whereby PLS negatively regulates ethylene responses to modulate cell division and expansion via downstream effects on microtubule cytoskeleton dynamics and auxin signaling, thereby influencing root growth and lateral root development. This mechanism involves a regulatory loop of auxin–ethylene interactions.

Journal Article Type Article
Publication Date Nov 1, 2006
Deposit Date May 17, 2007
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 18
Issue 11
Pages 3058-3072
DOI https://doi.org/10.1105/tpc.106.040790
Public URL https://durham-repository.worktribe.com/output/1552568