Skip to main content

Research Repository

Advanced Search

System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics

Zuleger, Nikolaj; Kelly, David A.; Richardson, A Christine; Kerr, Alistair R.W.; Goldberg, Martin W.; Goryachev, Andrew B.; Schirmer, Eric C.

System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics Thumbnail


Authors

Nikolaj Zuleger

David A. Kelly

A Christine Richardson

Alistair R.W. Kerr

Andrew B. Goryachev

Eric C. Schirmer



Abstract

The nuclear envelope contains >100 transmembrane proteins that continuously exchange with the endoplasmic reticulum and move within the nuclear membranes. To better understand the organization and dynamics of this system, we compared the trafficking of 15 integral nuclear envelope proteins using FRAP. A surprising 30-fold range of mobilities was observed. The dynamic behavior of several of these proteins was also analyzed after depletion of ATP and/or Ran, two functions implicated in endoplasmic reticulum–inner nuclear membrane translocation. This revealed that ATP- and Ran-dependent translocation mechanisms are distinct and not used by all inner nuclear membrane proteins. The Ran-dependent mechanism requires the phenylalanine-glycine (FG)-nucleoporin Nup35, which is consistent with use of the nuclear pore complex peripheral channels. Intriguingly, the addition of FGs to membrane proteins reduces FRAP recovery times, and this also depends on Nup35. Modeling of three proteins that were unaffected by either ATP or Ran depletion indicates that the wide range in mobilities could be explained by differences in binding affinities in the inner nuclear membrane.

Citation

Zuleger, N., Kelly, D. A., Richardson, A. C., Kerr, A. R., Goldberg, M. W., Goryachev, A. B., & Schirmer, E. C. (2011). System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Journal of Cell Biology, 193(1), 109-123. https://doi.org/10.1083/jcb.201009068

Journal Article Type Article
Online Publication Date Mar 28, 2011
Publication Date Mar 28, 2011
Deposit Date Apr 28, 2011
Publicly Available Date May 4, 2011
Journal Journal of Cell Biology
Print ISSN 0021-9525
Electronic ISSN 1540-8140
Publisher Rockefeller University Press
Peer Reviewed Peer Reviewed
Volume 193
Issue 1
Pages 109-123
DOI https://doi.org/10.1083/jcb.201009068

Files

Published Journal Article (3.5 Mb)
PDF

Copyright Statement
© 2011 Zuleger et al. This article is distributed under the terms of an Attribution–
Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication
date (see http://www.rupress.org/terms). After six months it is available under a
Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license,
as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).







You might also like



Downloadable Citations