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Interaction of PLS and PIN and hormonal crosstalk in Arabidopsis root development

Liu, J.L.; Mehdi, S.; Topping, J.; Friml, J.; Lindsey, K.

Interaction of PLS and PIN and hormonal crosstalk in Arabidopsis root development Thumbnail


Authors

S. Mehdi

J. Friml



Abstract

Understanding how hormones and genes interact to coordinate plant growth is a major challenge in developmental biology. The activities of auxin, ethylene, and cytokinin depend on cellular context and exhibit either synergistic or antagonistic interactions. Here we use experimentation and network construction to elucidate the role of the interaction of the POLARIS peptide (PLS) and the auxin efflux carrier PIN proteins in the crosstalk of three hormones (auxin, ethylene, and cytokinin) in Arabidopsis root development. In ethylene hypersignaling mutants such as polaris (pls), we show experimentally that expression of both PIN1 and PIN2 significantly increases. This relationship is analyzed in the context of the crosstalk between auxin, ethylene, and cytokinin: in pls, endogenous auxin, ethylene and cytokinin concentration decreases, approximately remains unchanged and increases, respectively. Experimental data are integrated into a hormonal crosstalk network through combination with information in literature. Network construction reveals that the regulation of both PIN1 and PIN2 is predominantly via ethylene signaling. In addition, it is deduced that the relationship between cytokinin and PIN1 and PIN2 levels implies a regulatory role of cytokinin in addition to its regulation to auxin, ethylene, and PLS levels. We discuss how the network of hormones and genes coordinates plant growth by simultaneously regulating the activities of auxin, ethylene, and cytokinin signaling pathways.

Journal Article Type Article
Publication Date Apr 5, 2013
Deposit Date Mar 18, 2013
Publicly Available Date Aug 22, 2013
Journal Frontiers in Plant Science
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 4
Article Number 75
DOI https://doi.org/10.3389/fpls.2013.00075
Keywords Hormonal crosstalk, Root development, Auxin flux, PIN proteins, PLS protein, Signaling network.
Public URL https://durham-repository.worktribe.com/output/1464545

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Published Journal Article (1.5 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Copyright: © 2013 Liu, Mehdi, Topping, Friml and Lindsey. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.






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