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Measurement of voltage-gated potassium currents in identified spinally projecting sympathetic neurons of the paraventricular nucleus

Barret-Jolley, R.; Pyner, S.; Coote, J.H.

Authors

R. Barret-Jolley

J.H. Coote



Abstract

The paraventricular nucleus (PVN) of the hypothalamus modulates cardiovascular function via a sub-population of neurones which project directly to sympathetic centres of the spinal cord. Identification and patch-clamp recording from these neurones is difficult, however, because of the complex organisation and neuronal heterogeneity of the PVN. We report here on methods for the in vitro recording of voltage-gated potassium channel (KV) currents from those neurones within the PVN which project to the intermediolateral column of the rat spinal cord, and are believed to directly modulate cardiovascular function. We show KV channel currents of spinally projecting neurones to be slowly inactivating (t100 ms) and weakly sensitive to TEA (Kd\10 mM). These methods will be useful for the study of KV and other ion channel modulation in spinally projecting neurones of the PVN.

Citation

Barret-Jolley, R., Pyner, S., & Coote, J. (2000). Measurement of voltage-gated potassium currents in identified spinally projecting sympathetic neurons of the paraventricular nucleus. Journal of Neuroscience Methods, 102(1), 25-33. https://doi.org/10.1016/s0165-0270%2800%2900271-5

Journal Article Type Article
Publication Date 2000-10
Deposit Date May 2, 2014
Journal Journal of Neuroscience Methods
Print ISSN 0165-0270
Electronic ISSN 1872-678X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 102
Issue 1
Pages 25-33
DOI https://doi.org/10.1016/s0165-0270%2800%2900271-5
Keywords PVN, Slice, Potassium current, KV, KV 1.1, KV 1.3, Fluorogold.
Public URL https://durham-repository.worktribe.com/output/1433553