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The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum

Wang, Pengwei; Hawkins, Timothy J.; Richardson, Christine; Cummins, Ian; Deeks, Michael J.; Sparkes, Imogen; Hawes, Chris; Hussey, Patrick J.

The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum Thumbnail


Authors

Pengwei Wang

Christine Richardson

Ian Cummins

Michael J. Deeks

Imogen Sparkes

Chris Hawes



Abstract

The cortical endoplasmic reticulum (ER) network in plants is a highly dynamic structure, and it contacts the plasma membrane (PM) at ER-PM anchor/contact sites. These sites are known to be essential for communication between the ER and PM for lipid transport, calcium influx, and ER morphology in mammalian and fungal cells. The nature of these contact sites is unknown in plants [1 and 2], and here, we have identified a complex that forms this bridge. This complex includes (1) NET3C, which belongs to a plant-specific superfamily (NET) of actin-binding proteins [3], (2) VAP27, a plant homolog of the yeast Scs2 ER-PM contact site protein [4 and 5], and (3) the actin and microtubule networks. We demonstrate that NET3C and VAP27 localize to puncta at the PM and that NET3C and VAP27 form homodimers/oligomers and together form complexes with actin and microtubules. We show that F-actin modulates the turnover of NET3C at these puncta and microtubules regulate the exchange of VAP27 at the same sites. Based on these data, we propose a model for the structure of the plant ER-PM contact sites.

Citation

Wang, P., Hawkins, T. J., Richardson, C., Cummins, I., Deeks, M. J., Sparkes, I., …Hussey, P. J. (2014). The Plant Cytoskeleton, NET3C, and VAP27 Mediate the Link between the Plasma Membrane and Endoplasmic Reticulum. Current Biology, 24(12), 1397-1405. https://doi.org/10.1016/j.cub.2014.05.003

Journal Article Type Article
Acceptance Date May 2, 2014
Online Publication Date Jun 5, 2014
Publication Date Jun 16, 2014
Deposit Date Jun 6, 2014
Publicly Available Date Jun 23, 2014
Journal Current Biology
Print ISSN 0960-9822
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 24
Issue 12
Pages 1397-1405
DOI https://doi.org/10.1016/j.cub.2014.05.003

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Copyright Statement
NOTICE: this is the author’s version of a work that was accepted for publication in Current Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Current Biology, 24, 12, 2014, 10.1016/j.cub.2014.05.003.







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