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Suppression of the homeobox gene HDTF1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton

Gao, W.; Long, L.; Xu, L.; Lindsey, K.; Zhang, X.; Zhu, L.

Suppression of the homeobox gene HDTF1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton Thumbnail


Authors

W. Gao

L. Long

L. Xu

X. Zhang

L. Zhu



Abstract

Development of pathogen-resistant crops, such as fungus-resistant cotton, has significantly reduced chemical application and improved crop yield and quality. However, the mechanism of resistance to cotton pathogens such as Verticillium dahliae is still poorly understood. In this study, we characterized a cotton gene (HDTF1) that was isolated following transcriptome profiling during the resistance response of cotton to V. dahliae. HDTF1 putatively encodes a homeodomain transcription factor, and its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea. To characterise the involvement of HDTF1 in the response to these pathogens, we used virus-induced gene silencing (VIGS) to generate HDTF1-silenced cotton. VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens. HDTF1 silencing resulted in activation of jasmonic acid (JA)-mediated signaling and JA accumulation. However, the silenced plants were not altered in the accumulation of salicylic acid (SA) or the expression of marker genes associated with SA signaling. These results suggest that HDTF1 is a negative regulator of the JA pathway, and resistance to V. dahliae and B. cinerea can be engineered by activation of JA signaling.

Journal Article Type Article
Acceptance Date Sep 21, 2015
Online Publication Date Nov 21, 2015
Publication Date May 1, 2016
Deposit Date Oct 2, 2015
Publicly Available Date Nov 21, 2016
Journal Journal of Integrative Plant Biology
Print ISSN 1672-9072
Electronic ISSN 1744-7909
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 58
Issue 5
Pages 503-513
DOI https://doi.org/10.1111/jipb.12432
Public URL https://durham-repository.worktribe.com/output/1421609

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Copyright Statement
This is the accepted version of the following article: Gao W, Long L, Xu L, Lindsey K, Zhang X, Zhu L ( 2016) Suppression of the homeobox gene HDTF1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton. Journal of Integrative Plant Biology, 58(5): 503-513, which has been published in final form at http://dx.doi.org/10.1111/jipb.12432. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.






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