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Small RNA and degradome profiling reveals a role for miRNAs and their targets in the developing fibers of Gossypium barbadense

Liu, N.; Tu, L.; Tang, W.; Gao, W.; Lindsey, K.; Zhang, X.

Small RNA and degradome profiling reveals a role for miRNAs and their targets in the developing fibers of Gossypium barbadense Thumbnail


Authors

N. Liu

L. Tu

W. Tang

W. Gao

X. Zhang



Abstract

microRNAs (miRNAs) are 20–24 nucleotide non-coding small RNAs that play important roles in plant development. The stages of cotton fiber development include initiation, elongation, secondary wall thickening (SWT) and maturation. We constructed seven fiber RNA libraries representing the initiation, elongation and SWT stages. In total, 47 conserved miRNA families and seven candidate miRNAs were profiled using small RNA sequencing. Northern blotting and real-time polymerase chain reaction (PCR) analyses revealed the dynamic expression of miRNAs during fiber development. In addition, 140 targets of 30 conserved miRNAs and 38 targets of five candidate miRNAs were identified through degradome sequencing. Analysis of correlated expression between miRNAs and their targets demonstrated that specific miRNAs suppressed the expression of transcription factors, SBP and MYB, a leucine-rich receptor-like protein kinase, a pectate lyase, α-tubulin, a UDP-glucuronic acid decarboxylase and cytochrome C oxidase subunit 1 to affect fiber development. Histochemical analyses detected the biological activity of miRNA156/157 in ovule and fiber development. Suppressing miRNA156/157 function resulted in the reduction of mature fiber length, illustrating that miRNA156/157 plays an essential role in fiber elongation.

Journal Article Type Article
Acceptance Date Aug 4, 2014
Online Publication Date Sep 15, 2014
Publication Date Oct 1, 2014
Deposit Date Nov 11, 2014
Publicly Available Date Jun 9, 2016
Journal Plant Journal
Print ISSN 0960-7412
Electronic ISSN 1365-313X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 80
Issue 2
Pages 331-344
DOI https://doi.org/10.1111/tpj.12636
Keywords Gossypium barbadense, Small RNA sequencing, Degradome sequencing, Fiber development, miRNA function verification.
Public URL https://durham-repository.worktribe.com/output/1420298

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Copyright Statement
This is the accepted version of the following article: Liu, N., Tu, L., Tang, W., Gao, W., Lindsey, K. and Zhang, X. (2014), Small RNA and degradome profiling reveals a role for miRNAs and their targets in the developing fibers of Gossypium barbadense. The Plant Journal, 80(2): 331-344, which has been published in final form at http://dx.doi.org/10.1111/tpj.12636. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.






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