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Spatiotemporal modelling of hormonal crosstalk explains the level and patterning of hormones and gene expression in Arabidopsis thaliana wildtype and mutant roots

Moore, S.; Zhang, X.; Mudge, A.; Rowe, J.; Topping, J.; Liu, J.; Lindsey, K.

Spatiotemporal modelling of hormonal crosstalk explains the level and patterning of hormones and gene expression in Arabidopsis thaliana wildtype and mutant roots Thumbnail


Authors

Simon Moore simon.moore@durham.ac.uk
PGR Student Doctor of Philosophy

X. Zhang

A. Mudge

J. Rowe



Abstract

Patterning in Arabidopsis root development is coordinated via a localized auxin concentration maximum in the root tip, requiring the regulated expression of specific genes. However, little is known about how hormone and gene expression patterning is generated. Using a variety of experimental data, we develop a spatiotemporal hormonal crosstalk model that describes the integrated action of auxin, ethylene and cytokinin signalling, the POLARIS protein, and the functions of PIN and AUX1 auxin transporters. We also conduct novel experiments to confirm our modelling predictions. The model reproduces auxin patterning and trends in wild-type and mutants; reveals that coordinated PIN and AUX1 activities are required to generate correct auxin patterning; correctly predicts shoot to root auxin flux, auxin patterning in the aux1 mutant, the amounts of cytokinin, ethylene and PIN protein, and PIN protein patterning in wild-type and mutant roots. Modelling analysis further reveals how PIN protein patterning is related to the POLARIS protein through ethylene signalling. Modelling prediction of the patterning of POLARIS expression is confirmed experimentally. Our combined modelling and experimental analysis reveals that a hormonal crosstalk network regulates the emergence of patterns and levels of hormones and gene expression in wild-type and mutants.

Journal Article Type Article
Acceptance Date Mar 20, 2015
Online Publication Date Apr 23, 2015
Publication Date Sep 1, 2015
Deposit Date Mar 20, 2015
Publicly Available Date Oct 15, 2015
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 207
Issue 4
Pages 1110-1122
DOI https://doi.org/10.1111/nph.13421
Keywords Hormonal crosstalk, Mathematical modelling, Mutant roots, Patterning of auxin, PIN proteins, PLS peptide, Root development.
Public URL https://durham-repository.worktribe.com/output/1413249

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Copyright Statement
This is an open access article under the terms of the Creative Commons Attribution License, which permits use,
distribution and reproduction in any medium, provided the original work is properly cited.







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