A recombinant fusion protein containing a spider toxin specific for the insect voltage-gated sodium ion channel shows oral toxicity towards insects of different orders
Yang, S.; Pyati, P.; Fitches, E.; Gatehouse, J.A.
Dr Elaine Fitches email@example.com
Recombinant fusion protein technology allows specific insecticidal protein and peptide toxins to display activity in orally-delivered biopesticides. The spider venom peptide δ-amaurobitoxin-PI1a, which targets insect voltage-gated sodium channels, was fused to the “carrier” snowdrop lectin (GNA) to confer oral toxicity. The toxin itself (PI1a) and an amaurobitoxin/GNA fusion protein (PI1a/GNA) were produced using the yeast Pichia pastoris as expression host. Although both proteins caused mortality when injected into cabbage moth (Mamestra brassicae) larvae, the PI1a/GNA fusion was approximately 6 times as effective as recombinant PI1a on a molar basis. PI1a alone was not orally active against cabbage moth larvae, but a single 30 μg dose of the PI1a/GNA fusion protein caused 100% larval mortality within 6 days when fed to 3rd instar larvae, and caused significant reductions in survival, growth and feeding in 4th – 6th instar larvae. Transport of fusion protein from gut contents to the haemolymph of cabbage moth larvae, and binding to the nerve chord, was shown by Western blotting. The PI1a/GNA fusion protein also caused mortality when delivered orally to dipteran (Musca domestica; housefly) and hemipteran (Acyrthosiphon pisum; pea aphid) insects, making it a promising candidate for development as a biopesticide.
Yang, S., Pyati, P., Fitches, E., & Gatehouse, J. (2014). A recombinant fusion protein containing a spider toxin specific for the insect voltage-gated sodium ion channel shows oral toxicity towards insects of different orders. Insect Biochemistry and Molecular Biology, 47, 1-11. https://doi.org/10.1016/j.ibmb.2014.01.007
|Journal Article Type||Article|
|Acceptance Date||Jan 21, 2014|
|Publication Date||Apr 1, 2014|
|Deposit Date||Mar 2, 2015|
|Publicly Available Date||May 8, 2015|
|Journal||Insect Biochemistry and Molecular Biology|
|Peer Reviewed||Peer Reviewed|
|Keywords||Neurotoxin, Insecticide, Recombinant protein expression system, Protein transport, Crop protection, Biotechnology.|
Published Journal Article
Publisher Licence URL
© 2014 The Authors. Published by Elsevier Ltd. Open access under CC BY license.
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