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ROS homeostasis regulates somatic embryogenesis via the regulation of auxin signaling in cotton

Zhou, T.; Yang, X.; Guo, K.; Deng, J.; Xu, J.; Gao, W.; Lindsey, K.; Zhang, X.

ROS homeostasis regulates somatic embryogenesis via the regulation of auxin signaling in cotton Thumbnail


Authors

T. Zhou

X. Yang

K. Guo

J. Deng

J. Xu

W. Gao

X. Zhang



Abstract

Somatic embryogenesis (S.E.) is a versatile model for understanding the mechanisms of plant embryogenesis and a useful tool for plant propagation. To decipher the intricate molecular program and potentially to control the parameters affecting the frequency of S.E., a proteomics approach based on two-dimensional gel electrophoresis (2-DE) combined with MALDI-TOF/TOF was used. A total of 149 unique differentially expressed proteins (DEPs) were identified at different stages of cotton S.E. compared with the initial control (0 h explants). The expression profile and functional annotation of these DEPs revealed that S.E. activated stress-related proteins, including several reactive oxygen species (ROS)-scavenging enzymes. Proteins implicated in metabolic, developmental, and reproductive processes were also identified. Further experiments were performed to confirm the role of ROS-scavenging enzymes, suggesting the involvement of ROS homeostasis during S.E. in cotton. Suppressing the expression of specifically identified GhAPX proteins resulted in the inhibition of dedifferentiation. Accelerated redifferentiation was observed in the suppression lines of GhAPXs or GhGSTL3 in parallel with the alteration of endogenous ascorbate metabolism and accumulation of endogenous H2O2 content. Moreover, disrupting endogenous redox homeostasis through the application of high concentrations of DPI, H2O2, BSO, or GSH inhibited the dedifferentiation of cotton explants. Mild oxidation induced through BSO treatment facilitated the transition from embryogenic calluses (ECs) to somatic embryos. Meanwhile, auxin homeostasis was altered through the perturbation of ROS homeostasis by chemical treatments or suppression of ROS-scavenging proteins, along with the activating/suppressing the transcription of genes related to auxin transportation and signaling. These results show that stress responses are activated during S.E. and may regulate the ROS homeostasis by interacting with auxin signaling.

Journal Article Type Article
Online Publication Date Apr 12, 2016
Publication Date Jun 1, 2016
Deposit Date Jun 15, 2016
Publicly Available Date Jun 16, 2016
Journal Molecular and Cellular Proteomics
Print ISSN 1535-9476
Electronic ISSN 1535-9484
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 15
Issue 6
Pages 2108-2124
DOI https://doi.org/10.1074/mcp.m115.049338
Public URL https://durham-repository.worktribe.com/output/1402171

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