Simple and versatile modifications allowing time gated spectral acquisition, imaging and lifetime profiling on conventional wide-field microscopes

Pal, Robert; Beeby, Andrew

Abstract

An inverted microscope has been adapted to allow time-gated imaging and spectroscopy to be carried out on samples containing responsive lanthanide probes. The adaptation employs readily available components, including a pulsed light source, time-gated camera, spectrometer and photon counting detector, allowing imaging, emission spectroscopy and lifetime measurements. Each component is controlled by a suite of software written in LabVIEW and is powered via conventional USB ports.

Citation

Pal, R., & Beeby, A. (2014). Simple and versatile modifications allowing time gated spectral acquisition, imaging and lifetime profiling on conventional wide-field microscopes. Methods and Applications in Fluorescence, 2(3), Article 037001. https://doi.org/10.1088/2050-6120/2/3/037001

• Journal Article Type: Article
• Acceptance Date: Jun 16, 2014
• Online Publication Date: Aug 22, 2014
• Publication Date: 2014-09
• Deposit Date: Mar 17, 2017
• Journal: Methods and Applications in Fluorescence
• Electronic ISSN: 2050-6120
• Publisher: IOP Publishing
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 3
• Article Number: 037001
• DOI: https://doi.org/10.1088/2050-6120/2/3/037001
• Public URL: https://durham-repository.worktribe.com/output/1390794