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The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor

Townsend, P.D.; Dixon, C.H.; Slootweg, E.S.; Sukarta, O.C.A.; Yang, A.W.H.; Hughes, T.R.; Sharples, G.J.; Palsson, L.-O.; Takken, F.L.W.; Goverse, A.; Cann, M.J.

The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor Thumbnail


Authors

P.D. Townsend

C.H. Dixon

E.S. Slootweg

O.C.A. Sukarta

A.W.H. Yang

T.R. Hughes

F.L.W. Takken

A. Goverse



Abstract

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato is further able to bind and distort double-stranded DNA. However, Rx1 host targets that support a role for Rx1 in transcriptional reprogramming at DNA are unknown. Here we report a functional interaction between Rx1 and NbGlk1, a Golden2- like transcription factor. Rx1 binds to NbGlk1 in vitro and in planta. NbGlk1 binds to known Golden2-like consensus DNA sequences. Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro. NbGlk1 activates cellular responses to potato virus X, whereas Rx1 associates with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX. This study provides new mechanistic insight into how an NLR can co-ordinate an immune signalling response at DNA following pathogen perception.

Citation

Townsend, P., Dixon, C., Slootweg, E., Sukarta, O., Yang, A., Hughes, T., …Cann, M. (2018). The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. Journal of Biological Chemistry, 293(9), 3218-3233. https://doi.org/10.1074/jbc.ra117.000485

Journal Article Type Article
Acceptance Date Dec 7, 2017
Online Publication Date Dec 7, 2017
Publication Date Mar 1, 2018
Deposit Date Jun 7, 2017
Publicly Available Date Mar 2, 2018
Journal Journal of Biological Chemistry
Print ISSN 0021-9258
Electronic ISSN 1083-351X
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 293
Issue 9
Pages 3218-3233
DOI https://doi.org/10.1074/jbc.ra117.000485

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Copyright Statement
This article is published under a Creative Commons CC-BY license.







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