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Long noncoding RNAs involved in resistance to Verticillium dahliae, a fungal disease in cotton

Zhang, L.; Wang, M.; Li, N.; Wang, H.; Qiu, P.; Hu, Q.; Pei, L.; Xu, Z.; Wang, T.; Gao, E.; Liu, J.; Liu, S.; Miao, Y.; Lindsey, K.; Tu, L.; Zhu, L.; Zhang, X.

Long noncoding RNAs involved in resistance to Verticillium dahliae, a fungal disease in cotton Thumbnail


Authors

L. Zhang

M. Wang

N. Li

H. Wang

P. Qiu

Q. Hu

L. Pei

Z. Xu

T. Wang

E. Gao

J. Liu

S. Liu

Y. Miao

L. Tu

L. Zhu

X. Zhang



Abstract

Long non-coding RNAs (lncRNAs) have several known functions in plant development, but their possible roles in responding to plant disease remain largely unresolved. In this study, we described a comprehensive disease-responding lncRNA profiles in defense against a cotton fungal disease Verticillium dahliae. We further revealed the conserved and specific characters of disease responding process between two cotton species. Conservatively for two cotton species, we found the expression dominance of induced lncRNAs in the Dt subgenome, indicating a biased induction pattern in the co-existing subgenomes of allotetraploid cotton. Comparative analysis of lncRNA expression and their proposed functions in resistant Gossypium barbadense cv. ‘7124′ versus susceptible G. hirsutum cv. ‘YZ1′ revealed their distinct disease response mechanisms. Species-specific (LS) lncRNAs containing more SNPs displayed a fiercer inducing level post infection than the species-conserved (core) lncRNAs. Gene Ontology enrichment of LS lncRNAs and core lncRNAs indicates distinct roles in the process of biotic stimulus. Further functional analysis showed that two core lncRNAs GhlncNAT-ANX2- and GhlncNAT-RLP7- silenced seedlings displayed an enhanced resistance towards V. dahliae and Botrytis cinerea, possibly associated with the increased expression of LOX1 and LOX2. This study represents the first characterization of lncRNAs involved in resistance to fungal disease and provides new clues to elucidate cotton disease response mechanism.

Journal Article Type Article
Acceptance Date Nov 1, 2017
Online Publication Date Dec 21, 2017
Publication Date Jun 1, 2018
Deposit Date Nov 8, 2017
Publicly Available Date Nov 28, 2017
Journal Plant Biotechnology Journal
Print ISSN 1467-7644
Electronic ISSN 1467-7652
Publisher Association of Applied Biologists
Peer Reviewed Peer Reviewed
Volume 16
Issue 6
Pages 1172-1185
DOI https://doi.org/10.1111/pbi.12861
Public URL https://durham-repository.worktribe.com/output/1371542

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.






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