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NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction

Wang, P.; Hussey, P.J.

NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction Thumbnail


Authors

P. Wang



Abstract

In plants movement of the endoplasmic reticulum (ER) is dependent on the actin cytoskeleton. However little is known about proteins that link the ER membrane and the actin cytoskeleton. Here we identified a novel protein, NETWORKED 3B (NET3B), which is associated with the ER and actin cytoskeleton in vivo. NET3B belongs to a superfamily of plant specific actin binding proteins, the NETWORKED family. NET3B associates with the actin cytoskeleton in vivo through an N-terminal NET actin binding (NAB) domain, which has been well-characterized in other members of the NET family. A three amino acid insertion, Val-Glu-Asp, in the NAB domain of NET3B appears to lower its ability to localize to the actin cytoskeleton compared with NET1A, the founding member of the NET family. The C-terminal domain of NET3B links the protein to the ER. Overexpression of NET3B enhanced the association between the ER and the actin cytoskeleton, and the extent of this association was dependent on the amount of NET3B available. Another effect of NET3B overexpression was a reduction in ER membrane diffusion. In conclusion, our results revealed that NET3B modulates ER and actin cytoskeleton interactions in higher plants.

Citation

Wang, P., & Hussey, P. (2017). NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction. Journal of Experimental Botany, 68(7), 1441-1450. https://doi.org/10.1093/jxb/erx047

Journal Article Type Article
Acceptance Date Jan 27, 2017
Online Publication Date Mar 28, 2017
Publication Date Mar 28, 2017
Deposit Date Feb 1, 2017
Publicly Available Date Feb 2, 2017
Journal Journal of Experimental Botany
Print ISSN 0022-0957
Electronic ISSN 1460-2431
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 68
Issue 7
Pages 1441-1450
DOI https://doi.org/10.1093/jxb/erx047

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Copyright Statement
© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which
permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.





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