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CRISPR/Cas system: recent advances and prospects for genome editing

Manghwar, H.; Lindsey, K.; Zhang, X.; Jin, S.

CRISPR/Cas system: recent advances and prospects for genome editing Thumbnail


Authors

H. Manghwar

X. Zhang

S. Jin



Abstract

Genome editing (GE) has revolutionized biological research through the new ability to precisely edit the genomes of living organisms. In recent years, various GE tools have been explored for editing simple and complex genomes. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has widely been used in GE due to its high efficiency, ease of use, and accuracy. It can be used to add desirable and remove undesirable alleles simultaneously in a single event. Here, we discuss various applications of CRISPR/Cas9 in a range of important crops, compare it with other GE tools, and review its mechanism, limitations, and future possibilities. Various newly emerging CRISPR/Cas systems, including base editing (BE), xCas9, and Cas12a (Cpf1), are also considered.

Journal Article Type Article
Publication Date Dec 31, 2019
Deposit Date Oct 1, 2019
Publicly Available Date Nov 26, 2019
Journal Trends in Plant Science
Print ISSN 1360-1385
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 24
Issue 12
Pages 1102-1125
DOI https://doi.org/10.1016/j.tplants.2019.09.006
Public URL https://durham-repository.worktribe.com/output/1290198

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