Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart
Shenton, F.C.; Campbell, T.; Jones, J.F.X.; Pyner, S.
Professor Susan Pyner firstname.lastname@example.org
Cardiac reflexes originating from sensory receptors in the heart ensure blood supply to vital tissues and organs in the face of constantly changing demands. Atrial volume receptors are mechanically sensitive vagal afferents which relay to the medulla and hypothalamus, affecting vasopressin release and renal sympathetic activity. To date, two anatomically distinct sensory endings have been identified which may subserve cardiac mechanosensation: end‐nets and flower‐spray endings. To map the distribution of atrial receptors in the subendocardial space, we have double‐labelled rat right atrial whole mounts for neurofilament heavy chain (NFH) and synaptic vesicle protein 2 (SV2) and generated high‐resolution maps of the rat subendocardial neural plexus at the cavo‐atrial region. In order to elucidate the nature of these fibres, double labelling with synaptophysin (SYN) and either NFH, calcitonin gene‐related peptide (CGRP), choline acetyltransferase (ChAT) or tyrosine hydroxylase (TH) was performed. The findings show that subendocardial nerve nets are denser at the superior cavo‐atrial junction than the mid‐atrial region. Adluminal plexuses had the finest diameters and stained positively for synaptic vesicles (SV2 and SYN), CGRP and TH. These plexuses may represent sympathetic post‐ganglionic fibres and/or sensory afferents. The latter are candidate substrates for type B volume receptors which are excited by stretch during atrial filling. Deeper nerve fibres appeared coarser and may be cholinergic (positive staining for ChAT). Flower‐spray endings were never observed using immunohistochemistry but were delineated clearly with the intravital stain methylene blue. We suggest that differing nerve fibre structures form the basis by which atrial deformation and hence atrial filling is reflected to the brain.
Shenton, F., Campbell, T., Jones, J., & Pyner, S. (2020). Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart. Journal of Anatomy, 238(1), 36-52. https://doi.org/10.1111/joa.13284
|Journal Article Type||Article|
|Acceptance Date||Jul 2, 2020|
|Online Publication Date||Aug 11, 2020|
|Deposit Date||Aug 4, 2020|
|Publicly Available Date||Aug 18, 2020|
|Journal||Journal of Anatomy|
|Peer Reviewed||Peer Reviewed|
Published Journal Article (Advance online version)
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Advance online version © 2020 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Published Journal Article
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