Anna Olerinyova
Mass Photometry of Membrane Proteins
Olerinyova, Anna; Sonn-Segev, Adar; Gault, Joseph; Eichmann, Cédric; Schimpf, Johannes; Kopf, Adrian H.; Rudden, Lucas S.P.; Ashkinadze, Dzmitry; Bomba, Radoslaw; Frey, Lukas; Greenwald, Jason; Degiacomi, Matteo T.; Steinhilper, Ralf; Killian, J. Antoinette; Friedrich, Thorsten; Riek, Roland; Struwe, Weston B.; Kukura, Philipp
Authors
Adar Sonn-Segev
Joseph Gault
Cédric Eichmann
Johannes Schimpf
Adrian H. Kopf
Lucas S.P. Rudden
Dzmitry Ashkinadze
Radoslaw Bomba
Lukas Frey
Jason Greenwald
Matteo Degiacomi matteo.t.degiacomi@durham.ac.uk
Part Time Teacher
Ralf Steinhilper
J. Antoinette Killian
Thorsten Friedrich
Roland Riek
Weston B. Struwe
Philipp Kukura
Abstract
Integral membrane proteins (IMPs) are biologically highly significant but challenging to study because they require maintaining a cellular lipid-like environment. Here, we explore the application of mass photometry (MP) to IMPs and membrane-mimetic systems at the single-particle level. We apply MP to amphipathic vehicles, such as detergents and amphipols, as well as to lipid and native nanodiscs, characterizing the particle size, sample purity, and heterogeneity. Using methods established for cryogenic electron microscopy, we eliminate detergent background, enabling high-resolution studies of membrane-protein structure and interactions. We find evidence that, when extracted from native membranes using native styrene-maleic acid nanodiscs, the potassium channel KcsA is present as a dimer of tetramers—in contrast to results obtained using detergent purification. Finally, using lipid nanodiscs, we show that MP can help distinguish between functional and non-functional nanodisc assemblies, as well as determine the critical factors for lipid nanodisc formation.
Citation
Olerinyova, A., Sonn-Segev, A., Gault, J., Eichmann, C., Schimpf, J., Kopf, A. H., …Kukura, P. (2021). Mass Photometry of Membrane Proteins. Chem, 7(1), 224-236. https://doi.org/10.1016/j.chempr.2020.11.011
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 11, 2020 |
Online Publication Date | Dec 14, 2020 |
Publication Date | Jan 14, 2021 |
Deposit Date | Dec 21, 2020 |
Publicly Available Date | May 4, 2021 |
Journal | Chem |
Print ISSN | 2451-9308 |
Publisher | Cell Press |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 1 |
Pages | 224-236 |
DOI | https://doi.org/10.1016/j.chempr.2020.11.011 |
Public URL | https://durham-repository.worktribe.com/output/1255110 |
Related Public URLs | https://ora.ox.ac.uk/objects/uuid:edb7aa7b-e5e2-4c83-b279-b7a4d056ccef |
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Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/
Copyright Statement
© 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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