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NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth

Duckney, Patrick; Kroon, Johan T.; Dixon, Martin R.; Hawkins, Timothy J.; Deeks, Michael J.; Hussey, Patrick J.

NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth Thumbnail


Authors

Martin R. Dixon

Michael J. Deeks



Abstract

We have recently characterised NET2A as a pollen‐specific actin‐binding protein which binds F‐actin at the plasma membrane of growing pollen tubes. However, the role of NET2 proteins in pollen development and fertilisation have yet to be elucidated. To further characterise the role of Arabidopsis NET2 proteins in pollen development and fertilisation, we analysed the subcellular localisation of NET2A over the course of pollen grain development, and investigated the role of the NET2 family using net2 loss‐of‐function mutants. We observed NET2A to localise to the F‐actin cytoskeleton in developing pollen grains as it underwent striking structural reorganisations at specific stages of development and during germination, and pollen tube growth. Furthermore, net2 loss‐of‐function mutants exhibited striking morphological defects in the early stages of pollen tube growth, arising from frequent alterations to pollen tube growth trajectory. We observed defects in the cortical actin cytoskeleton and actin‐driven subcellular processes in net2 mutant pollen tubes. We demonstrate that NET2 proteins are essential for normal actin‐driven pollen development highlighting an important role for the NET2 family members in regulating pollen tube growth during fertilisation.

Journal Article Type Article
Acceptance Date Mar 29, 2021
Online Publication Date Apr 16, 2021
Publication Date 2021-07
Deposit Date Apr 21, 2021
Publicly Available Date Jun 17, 2021
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 231
Issue 1
Pages 152-164
DOI https://doi.org/10.1111/nph.17391
Public URL https://durham-repository.worktribe.com/output/1249614

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Copyright Statement
© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.






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