Jack D. Fradgley
Targeted pH switched europium complexes monitoring receptor internalisation in living cells
Fradgley, Jack D.; Starck, Matthieu; Laget, Michel; Bourrier, Emmanuel; Dupuis, Elodie; Lamarque, Laurent; Trinquet, Eric; Zwier, Jurriaan M.; Parker, David
Authors
Matthieu Starck
Michel Laget
Emmanuel Bourrier
Elodie Dupuis
Laurent Lamarque
Eric Trinquet
Jurriaan M. Zwier
Professor David Parker david.parker@durham.ac.uk
Professor
Abstract
We report the design and evaluation of pH responsive luminescent europium(III) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.
Citation
Fradgley, J. D., Starck, M., Laget, M., Bourrier, E., Dupuis, E., Lamarque, L., …Parker, D. (2021). Targeted pH switched europium complexes monitoring receptor internalisation in living cells. Chemical Communications, 57(47), 5814-5817. https://doi.org/10.1039/d1cc01029h
| Journal Article Type | Article |
|---|---|
| Acceptance Date | May 4, 2021 |
| Online Publication Date | May 4, 2021 |
| Publication Date | 2021 |
| Deposit Date | Aug 25, 2021 |
| Publicly Available Date | Aug 25, 2021 |
| Journal | Chemical Communications |
| Print ISSN | 1359-7345 |
| Electronic ISSN | 1364-548X |
| Publisher | Royal Society of Chemistry |
| Peer Reviewed | Peer Reviewed |
| Volume | 57 |
| Issue | 47 |
| Pages | 5814-5817 |
| DOI | https://doi.org/10.1039/d1cc01029h |
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Copyright Statement
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.