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Single-cell RNA-seq reveals fate determination control of an individual fiber cell initiation in cotton (Gossypium hirsutum)

Qin, Y.; Sun, M.; Li, W.; Xu, M.; Shao, L.; Liu, Y.; Zhao, G.; Liu, Z.; Xu, Z.; You, J.; Ye, Z.; Xu, J.; Yang, X.; Wang, M.; Lindsey, K.; Zhang, X.; Tu, L.

Single-cell RNA-seq reveals fate determination control of an individual fiber cell initiation in cotton (Gossypium hirsutum) Thumbnail


Authors

Y. Qin

M. Sun

W. Li

M. Xu

L. Shao

Y. Liu

G. Zhao

Z. Liu

Z. Xu

J. You

Z. Ye

J. Xu

X. Yang

M. Wang

X. Zhang

L. Tu



Abstract

Cotton fiber is a unicellular seed trichome, and lint fiber initials per seed as a factor determines fiber yield. However, the mechanisms controlling fiber initiation from ovule epidermis are not understood well enough. Here, with single-cell RNA sequencing (scRNA-seq), a total of 14,535 cells were identified from cotton ovule outer integument of Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis and the day of anthesis). Three major cell types, fiber, non-fiber epidermis and outer pigment layer were identified and then verified by RNA in situ hybridization. A comparative analysis on scRNA-seq data between Xu142 and its fiberless mutant Xu142 fl further confirmed fiber cluster definition. The developmental trajectory of fiber cell was reconstructed, and fiber cell was identified differentiated at 1 day before anthesis. Gene regulatory networks at four stages revealed the spatiotemporal pattern of core transcription factors, and MYB25-like and HOX3 were demonstrated played key roles as commanders in fiber differentiation and tip-biased diffuse growth, respectively. A model for early development of a single fiber cell was proposed here, which sheds light on further deciphering mechanism of plant trichome and the improvement of cotton fiber yield.

Journal Article Type Article
Acceptance Date Aug 22, 2022
Online Publication Date Aug 29, 2022
Publication Date 2022-12
Deposit Date Aug 23, 2022
Publicly Available Date Sep 20, 2022
Journal Plant Biotechnology Journal
Print ISSN 1467-7644
Electronic ISSN 1467-7652
Publisher Association of Applied Biologists
Peer Reviewed Peer Reviewed
Volume 20
Issue 12
Pages 2372-2388
DOI https://doi.org/10.1111/pbi.13918
Public URL https://durham-repository.worktribe.com/output/1193749

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Publisher Licence URL
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Copyright Statement
© 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.






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