GhWRKY41 forms a positive feedback regulation loop and increases cotton defence response against Verticillium dahliae by regulating phenylpropanoid metabolism
Xiao, S.; Hu, Q.; Ye, Z.; Si, H.; Liu, S.; Zhang, X.; Wang, W.; Yu, Y.; Kong, J.; Klosterman, S.J.; Lindsey, K.; Aierxi, A.; Zhu, L.
Professor Keith Lindsey email@example.com
Despite the established significance of WRKY proteins and phenylpropanoid metabolism in plant immunity, how WRKY proteins modulate aspects of the phenylpropanoid pathway remains undetermined. To understand better the role of WRKY proteins in plant defence, we identified a cotton (Gossypium hirsutum) protein, GhWRKY41, that is, universally and rapidly induced in three disease-resistant cotton cultivars following inoculation with the plant pathogenic fungus, Verticillium dahliae. We show that overexpression of GhWRKY41 in transgenic cotton and Arabidopsis enhances resistance to V. dahliae, while knock-down increases cotton more susceptibility to the fungus. GhWRKY41 physically interacts with itself and directly activates its own transcription. A genome-wide chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq), in combination with RNA sequencing (RNA-seq) analyses, revealed that 43.1% of GhWRKY41-binding genes were up-regulated in cotton upon inoculation with V. dahliae, including several phenylpropanoid metabolism master switches, receptor kinases, and disease resistance-related proteins. We also show that GhWRKY41 homodimer directly activates the expression of GhC4H and Gh4CL, thereby modulating the accumulation of lignin and flavonoids. This finding expands our understanding of WRKY-WRKY protein interactions and provides important insights into the regulation of the phenylpropanoid pathway in plant immune responses by a WRKY protein.
Xiao, S., Hu, Q., Ye, Z., Si, H., Liu, S., Zhang, X., …Zhu, L. (2023). GhWRKY41 forms a positive feedback regulation loop and increases cotton defence response against Verticillium dahliae by regulating phenylpropanoid metabolism. Plant Biotechnology Journal, 21(5), 961-978. https://doi.org/10.1111/pbi.14008
|Journal Article Type||Article|
|Acceptance Date||Dec 24, 2022|
|Online Publication Date||Jan 12, 2023|
|Deposit Date||Apr 17, 2023|
|Publicly Available Date||Apr 17, 2023|
|Journal||Plant Biotechnology Journal|
|Publisher||Association of Applied Biologists|
|Peer Reviewed||Peer Reviewed|
Published Journal Article
Publisher Licence URL
© 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.<br /> <br /> This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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